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| Status |
Public on Oct 08, 2014 |
| Title |
Cyr61 promotes CD204 expression and migration of macrophages via MEK/Erk pathway in the microenvironment of human esophageal squamous cell carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The authors previously suggested that CD204 was a useful marker for tumor-associated macrophages (TAMs) of esophageal squamous cell carcinoma (ESCC). In this study, they discovered that within ESCC microenvironment not only cancer cells but also TAMs expressed cysteine-rich, angiogenic inducer, 61 (Cyr61), induced by conditioned media of ESCC cells. Levels of Cyr61 expression were associated with CD204+ macrophage infiltration in ESCC tissue. Cyr61 promoted CD204 expression and migration of macrophages via MEK/Erk pathway in vitro.
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| Overall design |
To identify the molecules specifically upregulated in TAM-like THP-1 cells, we performed a cDNA microarray analysis using RNAs from THP-1 stimulated with 200 nM TPA or with 200 nM TPA followed by 50% TE-8CM. THP-1 human acute monocytic leukemia cell line was treated with 200 nM 12-O-tetradecanoylphorbol-13-acetate (TPA) for 2 days to induce macrophage-like differentiation, then exposed to 50%Conditioned medium of TE-8 ESCC cell line (TE-8CM) for 2 days. Differentially expressed genes between MΦ-like and TAM-like THP-1 cells were analysed by cDNA microarray. TE-8CM was prepared by plating 5 × 106 tumor cells in 10 mL complete medium in 100-mm dishes for 24 hr, thereafter changing the medium to complete DMEM supplemented with 10% human AB serum instead of fetal bovine serum. After 2–3 days, the supernatants were harvested, centrifuged and stored in aliquots at −80°C.
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| Contributor(s) |
Shigeoka M, Yokozaki H |
| Citation(s) |
25620088 |
| Submission date |
Jul 16, 2014 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Manabu Shigeoka |
| E-mail(s) |
msk-shige@wi.kualnet.jp
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| Organization name |
Kobe University Graduate School of Medicine
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| Street address |
7-5-1 Kusunoki-cho, Chuo-ku
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| City |
Kobe |
| ZIP/Postal code |
650-0017 |
| Country |
Japan |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA255400 |
