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Series GSE59290 Query DataSets for GSE59290
Status Public on Feb 28, 2015
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary MiRNAs have been identfied to play an important role in cancer stem cells. MiR23b is differentially expressed in various forms of cancer including colorectal cancer as compared to their normal counterparts. MiR23b regulates various aspects of cell behaviour such as differentiation, apoptosis and motility. The goal of the study was to identify the novel role of miR23b in self-renewal property of colon cancer stem cells via regulation of its candidate target mRNAs. To address this aim, HT29 colon cancer cells were transfected with miR23b Precursor, Antimir and their respective controls. RNA SEQ analysis of the cells with altered levels of miR23b assisted in the identification of interesting mRNA targets influenced by miR23b expression and involved self-renewal pathways.
Overall design HT29 cells with altered levels of miR23b was subjected to RNA SEQ analysis to identify differential expresssion of mRNA targets of mIR23b
Contributor(s) Boman BM, Viswanathan V, Accerbi M, Schmidt S
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Submission date Jul 10, 2014
Last update date May 15, 2019
Contact name Bruce Boman
Phone 3186072764
Organization name Helen F Graham Cancer Center and Research Institute
Department CTCR
Lab suite 4300
Street address 4701, ogletown-stanton road
City Newark
State/province DE
ZIP/Postal code 19716
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (4)
GSM1432895 Precursor
GSM1432896 Precursor Control
GSM1432897 Antimir
BioProject PRJNA254974
SRA SRP044229

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59290_ANTI_isoform_exp.diff.txt.gz 7.1 Mb (ftp)(http) TXT
GSE59290_MIR_isoform_exp.diff.txt.gz 8.1 Mb (ftp)(http) TXT
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Processed data are available on Series record

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