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Series GSE59170 Query DataSets for GSE59170
Status Public on Mar 25, 2015
Title RNA expression profiling for Paf1 complex mutant strains [miRNA]
Organism Schizosaccharomyces pombe
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Small RNA molecules are well known for silencing gene expression post-transcriptionally, but they are also implicated in epigenetic regulation across kingdoms. However, the mechanisms of epigenetic gene silencing by small RNAs are mostly unknown. In addition, stably silencing genes de novo using synthetic small RNAs to trigger locus-independent heterochromatin formation is impossible. Using fission yeast, we show that RNA-directed heterochromatin formation is negatively controlled by the highly conserved RNA polymerase-associated factor 1 (Paf1) complex. Temporary expression of a synthetic hairpin RNA in Paf1 mutants triggers stable heterochromatin formation at homologous loci, effectively silencing genes in trans. This repressed state is propagated over many generations by continual production of secondary siRNAs, independently of the synthetic hairpin RNA. Our work uncovers a novel mechanism for small RNA-mediated epigenome regulation and highlights fundamental roles for the Paf1 complex and the RNA interference machinery in building epigenetic memory.
Overall design Small RNA was extracted from wild-type and mutant strains to compare miRNA gene expression.
Contributor(s) Kowalik KM, Shimada Y, Stadler M, Julia B, Bühler M
Citation(s) 25807481
Submission date Jul 07, 2014
Last update date May 15, 2019
Contact name Tim Roloff
Organization name FMI
Department Functional Genomics
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
Platforms (1)
GPL13988 Illumina HiSeq 2000 (Schizosaccharomyces pombe)
Samples (8)
GSM1429718 wt_spb464_1
GSM1429719 Paf1D_spb1953_1
GSM1429720 Leo1D_spb1955_1
This SubSeries is part of SuperSeries:
GSE59171 RNA expression profiling for Paf1 complex mutant strains
BioProject PRJNA254525
SRA SRP044125

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource 949.7 Kb (ftp)(http) TAB
GSE59170_RAW.tar 12.9 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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