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Status |
Public on Mar 03, 2015 |
Title |
Sequential centromere shift via de novo formation in maize |
Organism |
Zea mays |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
The ability of centromeres to alternate between active and inactive states indicates significant epigenetic elements controlling centromere assembly and centromere function. In maize (Zea mays), misdivision of the B chromosome centromere on a translocation with the short arm of chromosome 9 (TB-9Sb) can produce many variants with varying centromere sizes and centromeric DNA sequences. In derivatives of TB-9Sb, we found a de novo centromere on chromosome telo-3-3, which has no canonical centromeric repeat sequences. This centromere is derived from a 288-kb region on the short arm of chromosome 9, and is 19 megabases (Mb) removed from the translocation breakpoint of chromosome 9 in TB-9Sb. This centromere is much smaller than normal ones but can be maintained through meiosis. The functional B centromere in progenitor telo2-2 is deleted from telo3-3 but some B-repeat sequences remain. The de novo centromere of telo3-3 becomes inactive in three further derivatives with new centromeres being formed elsewhere on the chromosomes. One such de novo centromere contains only 200-kb CENH3 binding domain. This 200-kb centromere is located 3 Mb removed from the translocation breakpoint in a new location. The deleted B centromere in telo3-3 is activated in two derivatives. Our results suggest that de novo centromere formation is more common than previously thought and can persist on chromosomal fragments without a canonical centromere providing implications for karyotype evolution.
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Overall design |
ChIP-seq was carried out with anti-CENH3 antibodies using material from young leaves with control, telo3-3 and its derivate.
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Contributor(s) |
Han F, Birchler JA |
Citation(s) |
25733907 |
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Submission date |
Jul 07, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Handong Su |
E-mail(s) |
shandong@mail.hzau.edu.cn
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Phone |
+86-18810795796
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Organization name |
Huazhong Agricultural University
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Street address |
No.1,Shizishan Street ยท Hongshan District
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City |
Wuhan |
State/province |
Hubei |
ZIP/Postal code |
430070 |
Country |
China |
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Platforms (1) |
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Samples (6)
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Relations |
BioProject |
PRJNA254472 |
SRA |
SRP044103 |