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Series GSE59105 Query DataSets for GSE59105
Status Public on Apr 07, 2020
Title Affymetrix SNP array data for esophageal squamous cell carcinoma samples
Organism Homo sapiens
Experiment type SNP genotyping by SNP array
Genome variation profiling by SNP array
Summary Esophageal squamous cell carcinoma (ESCC) is an aggressive cancer with one of the highest world incidences in the Eastern Cape region of South Africa. Several genome wide studies have been performed on ESCC cohorts from Asian countries, North America, Malawi and other parts of the world but none has been conducted on ESCC tumors from South Africa to date, where the molecular pathology and etiology of this disease remains unclear. We report here tumor associated copy number changes observed in 51 ESCC patients’ samples from the Eastern Cape province of South Africa.

We extracted tumor DNA from 51 archived ESCC specimens and interrogated tumor associated DNA copy number changes using Affymetrix® 500K SNP array technology. The Genomic Identification of Significant Targets in Cancer (GISTIC) algorithm was applied to identify significant focal regions of gains and losses. Gains of the top recurrent cancer genes were validated by fluorescence in situ hybridization and their protein expression assessed by immunohistochemistry.

Twenty-three significant focal gains were identified across samples. Gains involving the CCND1, MYC, EGFR and JAG1 loci recapitulated those described in studies on Asian and Malawian cohorts. The two most significant gains involved the chromosomal sub-bands 3q28, encompassing the TPRG1 gene and 11q13.3 including the CTTN, PPFIA1and SHANK2 genes. There was no significant homozygous loss and the most recurrent hemizygous deletion involved the B3GAT1 gene on chromosome11q25. Focal gains on 11q13.3 in 37% of cases (19/51), consistently involved CTTN and SHANK2 genes. Twelve of these cases (23,5%), had a broader region of gain that also included the CCND1, FGF19, FGF4 and FGF3 genes. SHANK2 and CTTN are co-amplified in several cancers, these proteins interact functionally together and are involved in cell motility. Immunohistochemistry confirmed both Shank2 (79%) and cortactin (69%) protein overexpression in samples with gains of these genes. In contrast, cyclin D1 (65%) was moderately expressed in samples with CCND1 DNA gain.

This study reports copy number changes in a South African ESCC cohort and highlights similarities and differences with cohorts from Asia and Malawi. Our results strongly suggest a role for CTTN and SHANK2 in the pathogenesis of ESCC in South Africa.

Overall design Affymetrix SNP arrays were performed according to the manufacturer's instructions on DNA extracted from formalin-fixed paraffin-embedded tissue samples. Copy number analysis was performed on Affymetrix 500K SNP data for 51 esophageal squamous cell carcinomas. There were also 30 samples from normal formalin-fixed paraffin-embedded tissue samples from a matched population group that were used as a reference set for copy number inference.
Contributor(s) Brown J, Willem P, Stepien A
Citation(s) 32252688
Submission date Jul 06, 2014
Last update date Apr 20, 2020
Contact name Jacqueline Brown
Organization name National Health Laboratory Services
Department Molecular Medicine and Haematology
Lab Somatic Cell Genetics
Street address 7 York Rd Parktown
City Johannesburg
State/province Gauteng
ZIP/Postal code 2193
Country South Africa
Platforms (2)
GPL3718 [Mapping250K_Nsp] Affymetrix Mapping 250K Nsp SNP Array
GPL3720 [Mapping250K_Sty] Affymetrix Mapping 250K Sty2 SNP Array
Samples (162)
GSM1428582 Esophageal squamous carcinoma UROC16Nsp
GSM1428583 Esophageal squamous carcinoma UROC20Nsp
GSM1428584 Esophageal squamous carcinoma UROC22Nsp
BioProject PRJNA254426

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource 31.2 Mb (ftp)(http) TXT
GSE59105_FFPE_500K_ESCC_Partek_Raw.txt.gz 57.0 Mb (ftp)(http) TXT
GSE59105_RAW.tar 3.9 Gb (http)(custom) TAR (of CEL)
GSE59105_all.filelist.filtered.catted.seg.txt.gz 88.5 Kb (ftp)(http) TXT
GSE59105_readme.txt 1.2 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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