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Series GSE59065 Query DataSets for GSE59065
Status Public on May 29, 2015
Title Age-related profiling of DNA methylation in CD8+ T cells reveals changes in immune response and transcriptional regulator genes
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary Human ageing affects the immune system resulting in an overall decline in immunocompetence. Although all immune cells are affected during aging, the functional capacity of T cells is most influenced and is linked to decreased responsiveness to infections and impaired differentiation. We studied age-related changes in DNA methylation and gene expression in CD4+ and CD8+ T cells from younger and older individuals. We observed marked difference between T cell subsets, with increased number of methylation changes and higher methylome variation in CD8+ T cells with age. The majority of age-related hypermethylated sites were located at CpG islands of silent genes and enriched for repressive histone marks. Specifically, in CD8+ T cell subset we identified strong inverse correlation between methylation and expression levels in genes associated with T cell mediated immune response (LGALS1, IFNG, CCL5, GZMH, CCR7, CD27 and CD248) and differentiation (SATB1, TCF7, BCL11B and RUNX3). Our results thus suggest the link between age-related epigenetic changes and impaired T cell function.
Overall design The study includes 101 individuals, 50 young and 51 old, no replicates. From each individual there are CD4 and CD8 T-cell and peripheral blood samples extracted. The total number of samples is 296, of which 99 have CD4 T -cell, 100 have CD8 T-cell and 97 have peripheral blood data.
Contributor(s) Milani L, Peterson P
Citation(s) 26286994
Submission date Jul 03, 2014
Last update date Mar 22, 2019
Contact name Pärt Peterson
Organization name University of Tartu
Lab Laboratory of Molecular Pathology
Street address Ravila 19
City Tartu
ZIP/Postal code 50411
Country Estonia
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (296)
GSM1425464 SAMPLE_1_CD4
GSM1425465 SAMPLE_1
GSM1425466 SAMPLE_2_CD8
BioProject PRJNA254323

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59065_MatrixProcessed.csv.gz 940.9 Mb (ftp)(http) CSV
GSE59065_MatrixSignalIntensities.csv.gz 663.6 Mb (ftp)(http) CSV
GSE59065_RAW.tar 2.5 Gb (http)(custom) TAR (of IDAT)
Processed data are available on Series record

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