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| Status |
Public on May 29, 2015 |
| Title |
Age-related profiling of DNA methylation in CD8+ T cells reveals changes in immune response and transcriptional regulator genes |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Human ageing affects the immune system resulting in an overall decline in immunocompetence. Although all immune cells are affected during aging, the functional capacity of T cells is most influenced and is linked to decreased responsiveness to infections and impaired differentiation. We studied age-related changes in DNA methylation and gene expression in CD4+ and CD8+ T cells from younger and older individuals. We observed marked difference between T cell subsets, with increased number of methylation changes and higher methylome variation in CD8+ T cells with age. The majority of age-related hypermethylated sites were located at CpG islands of silent genes and enriched for repressive histone marks. Specifically, in CD8+ T cell subset we identified strong inverse correlation between methylation and expression levels in genes associated with T cell mediated immune response (LGALS1, IFNG, CCL5, GZMH, CCR7, CD27 and CD248) and differentiation (SATB1, TCF7, BCL11B and RUNX3). Our results thus suggest the link between age-related epigenetic changes and impaired T cell function.
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| Overall design |
The study includes 101 individuals, 50 young and 51 old, no replicates. From each individual there are CD4 and CD8 T-cell and peripheral blood samples extracted. The total number of samples is 296, of which 99 have CD4 T -cell, 100 have CD8 T-cell and 97 have peripheral blood data.
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| Contributor(s) |
Milani L, Peterson P |
| Citation(s) |
26286994 |
| Submission date |
Jul 03, 2014 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Pärt Peterson |
| E-mail(s) |
part.peterson@ut.ee
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| Organization name |
University of Tartu
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| Lab |
Laboratory of Molecular Pathology
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| Street address |
Ravila 19
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| City |
Tartu |
| ZIP/Postal code |
50411 |
| Country |
Estonia |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (296)
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| Relations |
| BioProject |
PRJNA254323 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE59065_MatrixProcessed.csv.gz |
940.9 Mb |
(ftp)(http) |
CSV |
| GSE59065_MatrixSignalIntensities.csv.gz |
663.6 Mb |
(ftp)(http) |
CSV |
| GSE59065_RAW.tar |
2.5 Gb |
(http)(custom) |
TAR (of IDAT) |
| Processed data are available on Series record |
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