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| Status |
Public on May 29, 2009 |
| Title |
Lipopolysaccharide-induced Changes in Gene Expression in Human Lung Microvascular Endothelial Cells over Time |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The vascular endothelium may play a role in the response to infectious agents and in the pathophysiology of disease processes resulting in capillary leak such as septic shock and acute respiratory distress syndrome. In order to study the effect of endotoxin on endothelial cell function, human lung microvascular endothelial cells in culture were exposed to lipopolysaccharide (LPS), 10 ng, for 4, 8, or 24 hours and changes in mRNA expression were studied using Affymetrix HG U133plus2 gene arrays. A principal components analysis revealed that LPS treatment was the primary source of variability in the data. LPS treatment of endothelial cells for 4, 8, or 24 hours resulted in the upregulation by two-fold or greater of 275, 260 and 141 genes respectively. LPS treatment resulted in the down regulation by 50% or greater of 176, 263 and 79 genes at 4, 8, or 24 hours respectively. Up regulated genes at 4 or 8 hours were enriched in those encoding for cytokines, secreted proteins, cell membrane proteins and proteins controlling signal transduction and transcriptional regulation. Down regulated genes at each of the time points included those coding for cell membrane proteins, transcriptional regulation and metabolism. At each time point, a significant proportion of the genes identified as changed were unique to that time point.
Keywords: Time course
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| Overall design |
In order to study the effect of endotoxin on endothelial cell function, human lung microvascular endothelial cells in culture were exposed to lipopolysaccharide (LPS), 10 ng, for 4, 8, or 24 hours and changes in mRNA expression were studied using Affymetrix HG U133plus2 gene arrays. Controls for each time point with no LPS exposure were also run. An N of 4 for each time point and treatment was used.
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| Contributor(s) |
Harry AC, Joubert D, Munson PJ, Barb J, Woszczek G, Nagineni S, Logun C, Suffredini AF, Danner RL, Shelhamer JH |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 20, 2006 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Anya Carmen Harry |
| E-mail(s) |
aharry@cc.nih.gov
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| Phone |
301-496-9320
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| Fax |
301-402-1213
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| Organization name |
NIH
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| Department |
CCMD
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| Street address |
10 Center Dr. MSC 1662 Bldg 10
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (24)
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| GSM114552 |
HMVEC-L stimulated with LPS for 4 hours L04R1 |
| GSM114553 |
HMVEC-L stimulated with LPS for 4 hours L04R2 |
| GSM114554 |
HMVEC-L stimulated with LPS for 4 hours L04R3 |
| GSM114555 |
HMVEC-L stimulated with LPS for 4 hours L04R4 |
| GSM114556 |
HMVEC-L stimulated with LPS for 8 hours L08R1 |
| GSM114557 |
HMVEC-L stimulated with LPS for 8 hours L08R2 |
| GSM114558 |
HMVEC-L stimulated with LPS for 8 hours L08R3 |
| GSM114559 |
HMVEC-L stimulated with LPS for 8 hours L08R4 |
| GSM114560 |
HMVEC-L stimulated with LPS for 24 hours L24R1 |
| GSM114561 |
HMVEC-L stimulated with LPS for 24 hours L24R2 |
| GSM114562 |
HMVEC-L stimulated with LPS for 24 hours L24R3 |
| GSM114563 |
HMVEC-L stimulated with LPS for 24 hours L24R4 |
| GSM114564 |
HMVEC-L stimulated without LPS for 4 hours c04R1 |
| GSM114565 |
HMVEC-L stimulated without LPS for 4 hours c04R2 |
| GSM114566 |
HMVEC-L stimulated without LPS for 4 hours c04R3 |
| GSM114567 |
HMVEC-L stimulated without LPS for 4 hours c04R4 |
| GSM114568 |
HMVEC-L stimulated without LPS for 8 hours c08R1 |
| GSM114569 |
HMVEC-L stimulated without LPS for 8 hours c08R2 |
| GSM114570 |
HMVEC-L stimulated without LPS for 8 hours c08R3 |
| GSM114571 |
HMVEC-L stimulated without LPS for 8 hours c08R4 |
| GSM114572 |
HMVEC-L stimulated without LPS for 24 hours c24R1 |
| GSM114573 |
HMVEC-L stimulated without LPS for 24 hours c24R2 |
| GSM114574 |
HMVEC-L stimulated without LPS for 24 hours c24R3 |
| GSM114575 |
HMVEC-L stimulated without LPS for 24 hours c24R4 |
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| Relations |
| BioProject |
PRJNA97301 |
| Supplementary data files not provided |
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