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Series GSE58775 Query DataSets for GSE58775
Status Public on Jun 13, 2015
Title Critical role of histone demethylase Jmjd3 in the regulation of CD4+ T cell differentiation
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Epigenetic factors have been implicated in the regulation of CD4(+) T-cell differentiation. Jmjd3 plays a role in many biological processes, but its in vivo function in T-cell differentiation remains unknown. Here we report that Jmjd3 ablation promotes CD4(+) T-cell differentiation into Th2 and Th17 cells in the small intestine and colon, and inhibits T-cell differentiation into Th1 cells under different cytokine-polarizing conditions and in a Th1-dependent colitis model. Jmjd3 deficiency also restrains the plasticity of the conversion of Th2, Th17 or Treg cells to Th1 cells. The skewing of T-cell differentiation is concomitant with changes in the expression of key transcription factors and cytokines. H3K27me3 and H3K4me3 levels in Jmjd3-deficient cells are correlated with altered gene expression through interactions with specific transcription factors. Our results identify Jmjd3 as an epigenetic factor in T-cell differentiation via changes in histone methylation and target gene expression.
Overall design ChIP-seq of histone modification marks H3K4me3 and H3K27me3 in WT and JMJD3 cKO mouse CD4+ T-cells
Contributor(s) Chepelev I
Citation(s) 25531312
Submission date Jun 24, 2014
Last update date May 15, 2019
Contact name Iouri Chepelev
Organization name US Department of Veterans Affairs Medical Center
Street address 3200 Vine St
City Cincinnati
State/province OH
ZIP/Postal code 45220
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (8)
GSM1419274 CD4_H3K4me3_WT_Rep1_GA1974
GSM1419275 CD4_H3K4me3_JMJD3-KO_Rep1_GA1975
GSM1419276 CD4_H3K27me3_WT_Rep1_GA1976
BioProject PRJNA253470
SRA SRP043531

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Supplementary file Size Download File type/resource
GSE58775_RAW.tar 233.8 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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