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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 07, 2015 |
| Title |
A Sleeping Beauty Forward Genetic Screen Identifies Genes Promoting Osteosarcoma Development and Metastasis |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
A Sleeping Beauty (SB) transposon forward genetic screen was performed to identify the genes that promote osteosarcoma (OS) development and metastasis. Mutagenesis induced OS in wild type mice and accelerated it on a Trp53 deficient background. Analysis of tumors demonstrated that Trp53 deficiency is correlated with genomic instability, which was virtually absent in tumors induced by SB mutagenesis alone. Metastases developed in a subset of animals and in nearly all cases were clonal related to primary tumors. Over 200 candidate genes were identified, many of which are altered in human cancers including OS. Signaling pathways enriched for candidate genes were also identified and a subset of these pathways and genes were functionally validated and represent new targets for OS treatment.
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| Overall design |
Bisulphite converted DNA from the 21 diagnosis osteosarcoma patients and 3 hOB cell line replicates were hybridised to the Illumina Infinium 450K Human Methylation Beadchip.
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| Contributor(s) |
Moriarity BS, Otto GM, Rahrmann EP, Rathe SK, Wolf NK, Manlove LA, LaRue RS, Temiz NA, Molyneux S, Choi K, Holly K, Sarver AL, Scott MC, Forster CL, Modiano JF, Khanna C, Hewitt S, Khokha R, Yang Y, Gorlick R, Dyer MA, Largaespada DA |
| Citation(s) |
25961939 |
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| Submission date |
Jun 24, 2014 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Chunxu Qu |
| E-mail(s) |
chunxu.qu@stjude.org
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| Phone |
9015952433
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| Organization name |
St Jude Children's Research Institute
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| Department |
Pathology
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| Lab |
Mullighan
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| Street address |
262 Danny Thomas Pl
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| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA253411 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58770_RAW.tar |
183.1 Mb |
(http)(custom) |
TAR |
| GSE58770_non_normalized.txt.gz |
50.5 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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