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Series GSE58770 Query DataSets for GSE58770
Status Public on Apr 07, 2015
Title A Sleeping Beauty Forward Genetic Screen Identifies Genes Promoting Osteosarcoma Development and Metastasis
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary A Sleeping Beauty (SB) transposon forward genetic screen was performed to identify the genes that promote osteosarcoma (OS) development and metastasis. Mutagenesis induced OS in wild type mice and accelerated it on a Trp53 deficient background. Analysis of tumors demonstrated that Trp53 deficiency is correlated with genomic instability, which was virtually absent in tumors induced by SB mutagenesis alone. Metastases developed in a subset of animals and in nearly all cases were clonal related to primary tumors. Over 200 candidate genes were identified, many of which are altered in human cancers including OS. Signaling pathways enriched for candidate genes were also identified and a subset of these pathways and genes were functionally validated and represent new targets for OS treatment.
Overall design Bisulphite converted DNA from the 21 diagnosis osteosarcoma patients and 3 hOB cell line replicates were hybridised to the Illumina Infinium 450K Human Methylation Beadchip.
Contributor(s) Moriarity BS, Otto GM, Rahrmann EP, Rathe SK, Wolf NK, Manlove LA, LaRue RS, Temiz NA, Molyneux S, Choi K, Holly K, Sarver AL, Scott MC, Forster CL, Modiano JF, Khanna C, Hewitt S, Khokha R, Yang Y, Gorlick R, Dyer MA, Largaespada DA
Citation(s) 25961939
Submission date Jun 24, 2014
Last update date Mar 22, 2019
Contact name Chunxu Qu
Phone 9015952433
Organization name St Jude Children's Research Institute
Department Pathology
Lab Mullighan
Street address 262 Danny Thomas Pl
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (24)
GSM1419204 genomic DNA from hOB cell ine, rep1
GSM1419205 genomic DNA from hOB cell ine, rep2
GSM1419206 genomic DNA from hOB cell ine, rep3
BioProject PRJNA253411

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58770_RAW.tar 183.1 Mb (http)(custom) TAR
GSE58770_non_normalized.txt.gz 50.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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