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Series GSE58747 Query DataSets for GSE58747
Status Public on Jul 16, 2014
Title Perturbed adult sperm methylome after in utero undernutrition is associated with transmission of metabolic disease
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary The pre and postnatal environment can affect both an individual’s risk of adult onset metabolic disease and that of subsequent generations. Although animal models and epidemiological data implicate epigenetic inheritance, little is known of the mechanisms involved. In a robust intergenerational model of developmental programming we demonstrate that the nutritional environment experienced in utero by F1 generation embryos alters the DNA methylome of the F1 adult male germ line in a locus-specific manner, without affecting overall methylation levels. Differentially methylated regions are mostly hypomethylated and are enriched in nucleosome retaining regions in adult sperm. A substantial fraction is resistant to early embryo methylation reprogramming, and thus have the potential to alter F2 generation development. Altered expression of transcripts neighbouring differentially methylated regions are evident in tissues of F2 offspring despite lack of persistence of differential methylation. Transmitted methylation variation in the germline at key regulatory loci may therefore contribute to the development of metabolic disease in the subsequent generation.
Overall design 2 biological replicates of pooled sperm samples for each control (C) or undernutrition (UN) model
Contributor(s) Radford EJ, Ito M, Shi H, Corish J, Yamazawa K, Isganaitis E, Seisenberger S, Hore T, Reik W, Erkek S, Peters A, Patti M, Ferguson-Smith AC
Citation(s) 25011554
Submission date Jun 23, 2014
Last update date May 15, 2019
Contact name Hui Shi
Organization name University of Cambridge
Department Department of Physiology, Development and Neuroscience
Street address Downing Street
City Cambridge
ZIP/Postal code CB2 3EG
Country United Kingdom
Platforms (2)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (4)
GSM1419028 meDIP-seq_data_CTRL1
GSM1419029 meDIP-seq_data_CTRL2
GSM1419030 meDIP-seq_data_UN1
BioProject PRJNA253334
SRA SRP043514

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Supplementary file Size Download File type/resource
GSE58747_RAW.tar 343.8 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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