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Series GSE58741 Query DataSets for GSE58741
Status Public on Nov 05, 2015
Title 3-anhydro-6-hydroxy-ophiobolin A inhibits the replication of influenza A virus in vitro and in vivo
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Influenza is a major cause of morbidity and mortality worldwide, and the emerging drug resistance poses an increasing challenge to the treatment of influenza virus infection. Therefore, the development of a novel antiviral drugs has become an urgent task to combat against the influenza viruses that are resistant to the current therapeutic treatment. Here, by screening a small molecule chemical compound library, we identified 3-anhydro-6-epi-ophiobolin A (named L435) as a potent anti-influenza agent. Mechanistically, L435 markedly reduced influenza virus replication in vitro and in vivo. Importantly, L435 treatment improved the survival of influenza-virus-infected mice, suggesting that L435 may be a novel therapeutic agent for treatment of influenza virus infections.
This microarray experiment was carried out to explore gene expression changes in influenza-virus-infected A549 cells after L435 treatment, and find out why L435 could inhibit the replication of influenza A virus.
Overall design Total RNAs were extracted from three different groups of A549 cells that were mock treated, or infected with WSN and treated with DMSO for 12h, or infected with WSN and treated with L435 for 12 h, using TRIzol reagent (Invitrogen, Carlsbad, CA). Three independent experiments were performed. Samples were amplified and labeled using the One-Color Quick Amp Labeling Kit (Agilent p/n 5190-2305).
Contributor(s) Chi X, Wang S, Luo X, Chen J
Citation(s) 26490959
Submission date Jun 23, 2014
Last update date Jan 09, 2018
Contact name xiaojuan chi
Phone 18046010428
Organization name Fujian Agriculture and Forestry University
Street address cangshan NO:15
City fuzhou
ZIP/Postal code 350002
Country China
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (9)
GSM1418977 Cell_WSN_L435_12h_rep1
GSM1418978 Cell_WSN_L435_12h_rep2
GSM1418979 Cell_WSN_L435_12h_rep3
BioProject PRJNA253311

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Supplementary file Size Download File type/resource
GSE58741_RAW.tar 111.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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