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Series GSE58740 Query DataSets for GSE58740
Status Public on Nov 12, 2014
Title Chromatin dynamics of p53 binding sites in IMR90
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary We report the chromatin modification dynamics at p53 binding sites upon treatment with DMSO or nutlin3-a (5uM) in IMR90 human lung fibroblasts using ChIP-seq and RNA-seq analyses.
We assessed the genomewide changes in H3, H3K4me3, H3K4me2, H3K4me1, H3K27ac, H4K16ac, RNA polymerase II, and p53 in response to p53 activation.
Overall design ChIP-seq of 8 histone modifications or proteins for 2 conditions in 1 cell line.
Contributor(s) Sammons M, Berger S
Citation(s) 25391375
Submission date Jun 23, 2014
Last update date May 15, 2019
Contact name Morgan Sammons
Organization name University of Pennsylvania
Street address 3400 Civic Center Blvd
City Philadelphia
State/province PA
ZIP/Postal code 19129
Country USA
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (22)
GSM1418957 H3 ChIP (DMSO)
GSM1418958 H3 ChIP (Nutlin-3a)
GSM1418959 H3K4me3 ChIP (DMSO)
BioProject PRJNA253315
SRA SRP043510

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58740_RAW.tar 8.9 Gb (http)(custom) TAR (of BED, BEDGRAPH, BIGWIG, TXT)
GSE58740_RNAseq_FPKM_DMSOvNutlin.txt.gz 659.3 Kb (ftp)(http) TXT
GSE58740_RNAseq_FPKM_DMSOvsNutlin_IMR90.txt.gz 733.2 Kb (ftp)(http) TXT
GSE58740_p53Nut_Enhancers_hg19.bed.gz 20.4 Kb (ftp)(http) BED
GSE58740_p53Nut_Protoenhancers_hg19.bed.gz 25.3 Kb (ftp)(http) BED
GSE58740_p53Nut_TSS_hg19.bed.gz 12.1 Kb (ftp)(http) BED
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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