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| Status |
Public on Feb 24, 2015 |
| Title |
Expression analysis of individual homoeologous wheat genome- and rye genome-specific transcripts in a 2BS.2RL wheat-rye translocation |
| Platform organism |
Triticum aestivum |
| Sample organisms |
Secale cereale; Triticum aestivum; Triticum urartu; Aegilops speltoides; Aegilops tauschii |
| Experiment type |
Expression profiling by array
|
| Summary |
To distinguish transcripts expressed from each of the three wheat genomes and those from the rye chromatins, genomic probes generated from diploid progenitors of wheat and rye were synthesized
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| Overall design |
Consensus sequences for probe design were generated from local unigene clusters. EST and cDNA sequences were downloaded from NCBI. A total of 52,589 pairs of probes were designed. Two 60 bp probes were designed from each consensus sequence. The array was manufactured by NimbleGen and synthesized on 12-plex arrays. Two independent biological replicates were used in separate hybridizations. Each biological replicate was a total RNA sample extracted from the whole tissue of two seedlings.
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| Contributor(s) |
Lee TG, Seo YW |
| Citation(s) |
25747040, 26484243 |
| |
| Submission date |
Jun 19, 2014 |
| Last update date |
Oct 21, 2015 |
| Contact name |
Tong Geon Lee |
| E-mail(s) |
agrospel@illinois.edu
|
| Organization name |
University of Illinois
|
| Street address |
1101 W Peabody
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA253144 |
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