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Series GSE58464 Query DataSets for GSE58464
Status Public on Jun 14, 2014
Title Rapid isolation of extracellular vesicles from cell culture and biological fluids using a synthetic peptide with specific affinity for heat shock proteins
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Background: Exosomes and extracellular vesicles (EVs) are increasingly recognized as important sources of biomarkers for disease study and diagnosis. Results: A synthetic peptide, Vn96, allows for capture of EVs from biological fluids using basic laboratory equipment. Conclusion: The Vn96-captured EVs are qualitatively equivalent or superior to exosomes isolated by ultracentrifugation. Significance: The Vn96 peptide provides an effective affinity-capture method for the isolation of EVs from biological fluids.
Overall design In order to compare different methods of exosome purification, we compared RNA content of exosomes purified with each method. We used two different breast cancer cell lines MCF7 and MDA-MB-231. We processed data in order to identify large RNAs as well as small RNA by using different methods for the alignment
Contributor(s) Ghosh A, Davey M, Chute IC, Griffiths SG, Lewis S, Chacko S, Barnett D, Crapoulet N, Fournier S, Joy A, Caissie MC, Ferguson AD, Daigle M, Meli VM, Lewis SM, Ouellette RJ
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Submission date Jun 13, 2014
Last update date May 15, 2019
Contact name Nicolas Crapoulet
Organization name Atlantic cancer research institute
Street address 35 Providence street
City Moncton
State/province NB
ZIP/Postal code e1c 8x3
Country Canada
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (5)
GSM1412080 MCF7_Exo
GSM1412081 MCF7_VN96
GSM1412082 MCF7_Ultra
BioProject PRJNA252722
SRA SRP043204

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Supplementary file Size Download File type/resource
GSE58464_RNA-seq_tophat_microrazers_results.txt.gz 3.4 Mb (ftp)(http) TXT
GSE58464_miRNA_exosome_after_R_TMM_normalization.txt.gz 5.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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