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| Status |
Public on Jun 11, 2014 |
| Title |
Cyclic mechanical stretch-induced gene expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
With gene expression profiling it was aimed to identify the differentially expressed genes associated with the regulation of the cytoskeleton to investigate the stretch-induced cell alignment mechanism. A whole genome microarray based analysis of the stretch-induced gene expression changes was done. Gene expression was measured at the beginning of the alignment process showing first reoriented cells after 5 h stretching and at the end after 24 h, where nearly all cells are aligned.
Cyclic mechanical stretching of cells results in cellular alignment perpendicular to the stretch direction regulating cellular response. This stress response is assumed to be an adaptation mechanism to reduce extensive stretching but also acts as architectural restructuring changing performance and biomechanics of the tissue. Gene expression profiling of control vs. stretched primary human dermal fibroblasts after 5 h and 24 h demonstrated the regulation of differentially expressed genes associated with metabolism, differentiation and morphology.
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| Overall design |
Primary human dermal fibroblasts from ten donors were cultured on Bioflex culture plates and stretched for 5h and 24 h or left untreated to avoid changes according to cell culturing. Each of the subject provided 4 samples (control/treated and 5hrs/24hrs) resulting in 40 samples total.
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| Contributor(s) |
Reichenbach M, Brückner B, Reuter H |
| Citation(s) |
26484124 |
| Submission date |
Jun 11, 2014 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Maria Reichenbach |
| Organization name |
Freie Universität Berlin
|
| Department |
Institute for Chemistry and Biochemistry
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| Lab |
Signal transduction
|
| Street address |
Thielallee 63
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA252431 |
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