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Series GSE58389 Query DataSets for GSE58389
Status Public on Jun 11, 2014
Title Cyclic mechanical stretch-induced gene expression
Organism Homo sapiens
Experiment type Expression profiling by array
Summary With gene expression profiling it was aimed to identify the differentially expressed genes associated with the regulation of the cytoskeleton to investigate the stretch-induced cell alignment mechanism. A whole genome microarray based analysis of the stretch-induced gene expression changes was done. Gene expression was measured at the beginning of the alignment process showing first reoriented cells after 5 h stretching and at the end after 24 h, where nearly all cells are aligned.
Cyclic mechanical stretching of cells results in cellular alignment perpendicular to the stretch direction regulating cellular response. This stress response is assumed to be an adaptation mechanism to reduce extensive stretching but also acts as architectural restructuring changing performance and biomechanics of the tissue. Gene expression profiling of control vs. stretched primary human dermal fibroblasts after 5 h and 24 h demonstrated the regulation of differentially expressed genes associated with metabolism, differentiation and morphology.
Overall design Primary human dermal fibroblasts from ten donors were cultured on Bioflex culture plates and stretched for 5h and 24 h or left untreated to avoid changes according to cell culturing. Each of the subject provided 4 samples (control/treated and 5hrs/24hrs) resulting in 40 samples total.
Contributor(s) Reichenbach M, Brückner B, Reuter H
Citation(s) 26484124
Submission date Jun 11, 2014
Last update date Nov 27, 2018
Contact name Maria Reichenbach
Organization name Freie Universität Berlin
Department Institute for Chemistry and Biochemistry
Lab Signal transduction
Street address Thielallee 63
City Berlin
ZIP/Postal code 14195
Country Germany
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (40)
GSM1409722 co_5h_392
GSM1409723 co_24h_392
GSM1409724 co_5h_420
BioProject PRJNA252431

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Supplementary file Size Download File type/resource
GSE58389_RAW.tar 495.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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