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Series GSE58388 Query DataSets for GSE58388
Status Public on Nov 06, 2014
Title Contribution of Veillonella parvula to Pseudomonas aeruginosa mediated pathogenicity in a murine tumor model system
Organisms Mus musculus; Veillonella parvula DSM 2008; Pseudomonas aeruginosa PA14
Experiment type Expression profiling by high throughput sequencing
Summary We investigated the specific interactions of the most dominant bacterial CF-pathogen, Pseudomonas aeruginosa, and the anaerobic bacterium Veilllonella parvula, that has been recovered at comparable cell numbers in the respiratory tract of CF patients. We used our recently established in-vivo murine tumor model to investigate mutual influences of the two pathogens during a biofilm-associated infection process. We found that although P. aeruginosa and V. parvula colonized distinct niches within the tumor, in mice that were co-infected with both bacterial species significant higher cell numbers of P. aeruginosa were recovered from the tumor tissue. Concordantly, in vivo transcriptional profiling implied that the presence of V. parvula supports P. aeruginosa growth at the infected host site, and the higher P. aeruginosa load correlated with clinical deterioration.
Overall design We cultivated P. aeruginosa PA14 and V. parvula DSM No.:2008 in mono- and co-cultures in vivo using an established murine tumor model. Corresponding in vitro samples were generated under anaerobe growth conditions.
Contributor(s) Pustelny C, Komor U, Pawar V, Lorenz A, Moter A, Eckweiler D, Grothe C, Lünsdorf H, Weiss S, Häussler S
Citation(s) 25385800
Submission date Jun 11, 2014
Last update date May 15, 2019
Contact name Denitsa Eckweiler
Organization name Technische Universität Braunschweig
Department Institute of Microbiology
Lab Prof. Dieter Jahn
Street address Spielmannstr. 7
City Braunschweig
State/province Lower Saxony
ZIP/Postal code D-38106
Country Germany
Platforms (4)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL18780 Illumina Genome Analyzer IIx (Pseudomonas aeruginosa PA14)
GPL18781 Illumina Genome Analyzer IIx (Veillonella parvula DSM 2008)
Samples (12)
GSM1409710 Pure_invitro_PA14
GSM1409711 Pure_invitro_Vpa
GSM1409712 Mix_invitro
BioProject PRJNA252432
SRA SRP043109

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58388_RAW.tar 470.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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