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Series GSE58374 Query DataSets for GSE58374
Status Public on Mar 16, 2015
Title SMO variants explain the majority of drug resistance in basal cell carcinoma [exome-seq]
Organism Homo sapiens
Experiment type Other
Summary Advanced basal cell carcinomas (BCCs) frequently acquire resistance to Smoothened (SMO) inhibitors through unknown mechanisms, providing a unique opportunity to study human tumor evolution. Here, we identify SMO mutations in 50% (22/44) of resistant BCCs compared with 5.6% (2/36) of untreated BCCs (p<0.0001), and show that these mutations maintain Hedgehog signaling in the presence of SMO inhibitors. Alterations include four ligand binding pocket (LBP) mutations that define sites of inhibitor binding and four variants that confer constitutive activity and inhibitor resistance, thus defining pivotal residues of SMO that ensure receptor autoinhibition. Finally, we show that both classes of SMO variants respond to the aPKC-ι/λ inhibitor PSI and GLI2 antagonist ATO that operate downstream of SMO.
Overall design Identification of somatic mutations in 14 resistant BCCs along with matched adjacent normal skin using exome sequencing.
Contributor(s) Atwood SX, Sarin KY, Li J, Kim G, Rezaee M, Ally MS, Kim J, Yao C, Whitson RJ, Chang AS, Oro AE, Tang JY
Citation(s) 25759020
Submission date Jun 10, 2014
Last update date May 15, 2019
Contact name Jiang Li
Phone 6507258839
Organization name Stanford University
Department Dermatology
Lab Tony Oro
Street address 269 Campus Drive, Stanford University
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (20)
GSM1409314 DNASeq-BCC1-control
GSM1409315 DNASeq-BCC1-tumor
GSM1409316 DNASeq-BCC2-tumor
This SubSeries is part of SuperSeries:
GSE58377 SMO variants explain the majority of drug resistance in basal cell carcinoma
BioProject PRJNA252376
SRA SRP043084

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