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Series GSE58152 Query DataSets for GSE58152
Status Public on Dec 31, 2014
Title Transcriptome of CD4+ T cell subsets
Organism Mus musculus
Experiment type Expression profiling by array
Summary We compared the transcriptome of ex vivo-isolated murine naive CD4+ T cells, Th1 cells and Th17 cells by microarray.
 
Overall design CD4+ T effector cells were prepared ex vivo, stimulated with PMA/Ionomycin, subjected to a comercially available cytokine secretion kit (IL-17A and IFNg), stained by adding fluorescence-labeled antibodies against CD3, CD4 and CD45RB and sorted by flow cytometry. We sorted naive CD4+ T cells (CD3+CD4+CD45RB_high), Th1 cells (CD3+CD4+CD45RB_low_IFNg+IL17A-) and Th17 cells (CD3+CD4+CD45RB_low_IFNg-IL17A+).
 
Contributor(s) Floess S, Yang B, Geffers R
Citation(s) 25593324
Submission date Jun 02, 2014
Last update date Nov 01, 2017
Contact name Robert Geffers
E-mail(s) robert.geffers@helmholtz-hzi.de
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
 
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (9)
GSM1402510 Tnaive_1
GSM1402511 Tnaive_2
GSM1402512 Tnaive_3
Relations
BioProject PRJNA251397

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58152_RAW.tar 19.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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