GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE58116 Query DataSets for GSE58116
Status Public on Jun 26, 2014
Title Helminth Infection Reactivates Latent γ-herpesvirus Via Cytokine Competition at a Viral Promoter
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Mammals are co-infected by multiple pathogens that interact through unknown mechanisms. We found that helminth infection, characterized by the induction of the cytokine interleukin-4 (IL-4) and the activation of the transcription factor Stat6, reactivated murine gammaherpesvirus infection in vivo. IL-4 promoted viral replication and blocked the antiviral effects of interferon-g (IFNg) by inducing Stat6 binding to the promoter for an important viral transcriptional transactivator. IL-4 also reactivated human Kaposi's sarcoma associated herpesvirus from latency in cultured cells. Exogenous IL-4 plus blockade of IFNg reactivated latent murine gammaherpesvirus infection in vivo, suggesting a ‘two-signal’ model for viral reactivation. Thus chronic herpesvirus infection, a component of the mammalian virome, is regulated by the counterpoised actions of multiple cytokines on viral promoters that have evolved to sense host immune status.
Overall design All of the RNA from virus-pos cells and 50 ng of RNA from virus-neg cells was prepared for RNA-seq using ScriptSeq v2 RNA-seq library preparation kit (Epicentre). Index Primers (Epicentre) were added and samples underwent Duplex-Specific thermostable nuclease (DSN) (Evrogen) treatment to remove ribosomal RNA. Samples were pooled and sequenced on HiSeq.
Contributor(s) Artyomov MN
Citation(s) 24968940
Submission date May 30, 2014
Last update date May 15, 2019
Contact name Maxim N. Artyomov
Organization name Washington University in St.Louis
Department Immunology&Pathology
Street address 660 S. Euclid Avenue, Campus Box 8118
City St.Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM1400986 TR192 Macrophage virus Negative rep 1
GSM1400987 TR192 Mac virus Positive rep 1
GSM1400988 TR192 Macrophage virus Negative rep 2
BioProject PRJNA248947
SRA SRP042593

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58116_Barcodes.txt.gz 157 b (ftp)(http) TXT
GSE58116_Gene_expression_FPKM.table.diffexpr.txt.gz 290.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap