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Series GSE58066 Query DataSets for GSE58066
Status Public on Jan 14, 2015
Title Dnmt3L-histone H3 binding is necessary for male fertility and non-CG methylation
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary Patterns of DNA methylation are established during gametogenesis. The catalytically-inactive adaptor Dnmt3L is crucial to ensure this occurs correctly. In vitro, Dnmt3L binds to the N terminal tail of histone H3 but the function of this interaction during development is unknown. Here we show that Dnmt3L-histone H3 interaction is necessary for spermatogenesis. Mutant animals exhibit reduced fertility, defective methylation establishment at retrotransposons coupled with their reactivation and meiotic catastrophe. The spermatogonial stem cell pool is also defective, with mutant cells displaying marked changes in gene expression. Genome-wide methylation analysis reveals reductions in CG methylation as well as severe loss of non-CG methylation suggesting that non-CG methylation is specifically sensitive to the ability of Dnmt3L to bind histone H3.
Overall design MethylC-Seq of wild-type and Dnmt3LA/A 1dpp prospermatagonia
Contributor(s) Schmitz RJ, Ooi SK
Citation(s) 25683717
Submission date May 29, 2014
Last update date May 15, 2019
Contact name Robert J Schmitz
Organization name University of Georgia
Department Genetics
Street address B416 Davison Life Sciences
City Athens
State/province GA
ZIP/Postal code 30602
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (2)
GSM1400326 Wild type
GSM1400327 DNMT3 A/A
BioProject PRJNA248793
SRA SRP042360

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58066_RAW.tar 7.8 Gb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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