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Series GSE57767 Query DataSets for GSE57767
Status Public on May 20, 2014
Title Epigenome analysis of normal and preeclamptic placenta samples
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary Preeclampsia (PE), a hypertensive disorder of pregnancy, is hypothesized to be associated with, if not mechanistically related to abnormal placental function. However, the exact mechanisms regulating the pathogenesis of PE remain unclear. While many studies have investigated changes in gene expression in the PE placenta, the role of epigenetics in PE associated placental dysfunction remains unclear. Using the genome-wide Illumina Infinium Methylation 450 BeadChip array, we analyzed gene-specific alterations in DNA methylation in placental biopsies collected from normal pregnant women delivering at term (n=14), with term PE (≥37 weeks; n=19) or with preterm PE (<37 weeks, n=12). Of the 485,582 gene loci on the array, compared to controls, 229 loci were differentially methylated in PE placentas and 3411 loci were differentially methylated in preterm PE (step up p-value <0.05 and >5% methylation difference). Functional annotation of the differentially methylated genes in preterm PE placentas revealed a 32 gene cluster in the cadherin and cell adhesion functional groups (Benjamini p<0.00001). Hypermethylation of CDH11 (p=0.0143), COL5A1 (p=0.0127) and TNF (p=0.0098) and hypomethylation of NCAM1 (p=0.0158) was associated with altered mRNA expression in preterm PE placentas. These studies demonstrate aberrant methylation, correlating with disease severity, in PE placentas.
Overall design Bisulphite converted DNA from the 45 samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip v1.2
Contributor(s) Anton L, Elovitz MA
Citation(s) 24963923
Submission date May 17, 2014
Last update date Mar 22, 2019
Contact name Lauren Anton
Organization name University of Pennsylvania
Department Obstetrics and Gynecology
Lab Michal Elovitz
Street address 421 Curie Blvd, 1349 BRB II/III
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (45)
GSM1388230 genomic DNA from placenta 4001
GSM1388231 genomic DNA from placenta 4002
GSM1388232 genomic DNA from placenta 4003
BioProject PRJNA248043

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57767_RAW.tar 183.1 Mb (http)(custom) TAR
GSE57767_signal_intensities.txt.gz 185.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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