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Series GSE57680 Query DataSets for GSE57680
Status Public on Nov 01, 2014
Title RNA-sequencing of luminal epithelium and stroma cells isolated by LCM from pseudopregnant day 4 Msx1/Msx2 knockout and control uteri
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We recently demonstrated that Msx genes, which encode muscle segment homeobox (Msh) transcription factors, regulate the transition of the luminal epithelium from high to low apicobasal polarity that is critical for implantation. In Msx deficient uteri (Msx1d/d/Msx2d/d), apicobasal polarity remains high and implantation fails to occur. However, information on the underlying molecular mechanism of Msx-dependent regulation of epithelial polarity, and the nature of epithelial-mesenchymal interactions that are characteristic of Msx genes, remain limited. In this study, we analyzed gene expression by RNA-sequencing in the luminal epithelium and stroma isolated by laser capture microdissection (LCM) on day 4 of pseudopregnancy in Msx1f/f/Msx2f/f and Msx1d/d/Msx2d/d uteri. We found upregulation of extracellular matrix components in the stroma and downregulation of immunity-related genes in both the luminal epithelium and stroma isolated from Msx1d/d/Msx2d/d mice. In addition, tight junction protein Claudin 1 and small proline-rich protein (Sprr2) were substantially upregulated in Msx1d/d/Msx2d/d epithelia.
Overall design mRNA profiles of luminal epithelium and stroma from a wildtype Msx1f/f/Msx2f/f and a uterine-specific knockout Msx1d/d/Msx2d/d mouse were asessed by RNA-sequencing with the Illumina HiSeq 1500 system
Contributor(s) Park CB, Sun X, Potter SS, Dey SK
Citation(s) 26667042
Submission date May 14, 2014
Last update date Jul 26, 2019
Contact name SK Dey
Phone 513-803-1158
Organization name Cincinnati Children's Hospital
Department Reproductive Sciences
Street address 3333 Burnet Ave
City Cincinnti
ZIP/Postal code 45229
Country USA
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (4)
GSM1386589 Msx1-2 (d-d) KO - LCM RNA-Seq - Luminal Epithelium
GSM1386590 Msx1-2 (f-f) WT - LCM RNA-Seq - Luminal Epithelium
GSM1386591 Msx1-2 (d-d) KO - LCM RNA-Seq - Stroma
BioProject PRJNA247672
SRA SRP041994

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57680_RPKM_luminal_epithelium.txt.gz 794.4 Kb (ftp)(http) TXT
GSE57680_RPKM_stroma.txt.gz 834.7 Kb (ftp)(http) TXT
GSE57680_Supplemental_Table_S1.xlsx 21.1 Kb (ftp)(http) XLSX
GSE57680_Supplemental_Table_S2_GO_LE_all_list.xlsx 19.2 Kb (ftp)(http) XLSX
GSE57680_Supplemental_Table_S3_GO_ST_all_list.xlsx 98.3 Kb (ftp)(http) XLSX
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Processed data are available on Series record

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