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Series GSE5764 Query DataSets for GSE5764
Status Public on Mar 16, 2007
Title Analysis of microdissected invasive lobular and ductal breast carcinomas in relation to normal ductal and lobular cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The aim of our study was to identify gene expression profiles of ductal and lobular carcinomas in relation to normal ductal and lobular cells. We examined ten mastectomy specimens from postmenopausal breast cancer patients. Ductal and lobular tumor and normal cells were microdissected from cryosections. Fifty nanograms of total RNA were amplified and labeled by PCR and in vitro transcription. GCOS pairwise comparison algorithm and rank products have identified multiple genes that are differentially expressed in comparisons between ductal and lobular tumor and normal cell types. The results suggest that these genes are involved in epithelial-mesenchymal transition, TGFbeta and Wnt signaling. These changes are present in both tumor types but appear to be more prominent in lobular carcinomas.
Keywords: cell type comparison
 
Overall design Ten surgical specimens obtained by mastectomy from postmenopausal patients with invasive ductal (IDC) and lobular breast (ILC) carcinomas were investigated. Of these, 5 were IDCs and 5 were ILCs. Tumor and normal tissues from the same mammary gland were identified by an experienced pathologist, snap-frozen in liquid nitrogen and stored at -80ÂșC for further analysis. Microdissection, RNA isolation, amplification, labeling and microarray analysis are described in sample definitions. Samples from particular cell types (normal ductal, normal lobular, tumor ductal, tumor lobular - 10, 10, 5, 5 samples, respectively) were considered as biological replicates and were compared in between.
 
Contributor(s) Turashvili G, Bouchal J, Baumforth K, Wei W, Dziechciarkova M, Ehrmann J, Klein J, Fridman E, Skarda J, Hajduch M, Murray P, Kolar Z
Citation(s) 17389037
Submission date Sep 05, 2006
Last update date Mar 25, 2019
Contact name Jan Bouchal
E-mail(s) jan.bouchal@gmail.com
Phone +420585639570
Organization name Palacky University
Department Department of Clinical and Molecular Pathology
Lab Laboratory of Molecular Pathology
Street address Hnevotinska 3
City Olomouc
ZIP/Postal code CZ-77900
Country Czech Republic
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (30)
GSM134584 6ILC_normal_ductal
GSM134586 6ILC_normal_lobular
GSM134587 6ILC_tumor_lobular
Relations
BioProject PRJNA97101

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5764_RAW.tar 237.6 Mb (http)(custom) TAR (of CEL, EXP)

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