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Series GSE57602 Query DataSets for GSE57602
Status Public on Jul 14, 2014
Title RNAseq analysis of mouse lung transcriptome from allergic and non-allergic mice prior to and following lung Klebsiella pneumoniae infection
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We have demonstrated that allergic airway inflammation (induced by an ovalbumin sensitization and aerosol challenge protocol) decreases lung bacterial burden following lung infection with Klebsiella pneumoniae. The goals of this study are to indentify novel targets that are expressed during allergic airway inflammation in this model that contribute to enhanced lung bacterial immunity.
Overall design We isolated total RNA from the lungs of 4 groups of mice at both 0 hours (pre-infection) and 6 hours post-infection. WT and STAT6KO (BALB/c) mice were intraperitoneally sensitzed with alum or ovalbumin (OVA)-alum on day -18. Alum injected mice were not subsequently exposed to OVA aerosol. OVA-alum injected mice underwent aerosol sensitization on days -4, -3, -2, and -1. On day 0, four groups of mice were harvested (pre-infection). These included WT-ALUM, WT-OVA, STAT6KO-ALUM, and STAT6KO-OVA. On day 0, four groups of mice were infected with 10^4cfu of Klebsiella and then lungs were removed at 6 hours post-infection. These groups included WT-ALUM-KP, WT-OVA-KP, STAT6KO-ALUM-KP, and STAT6KO-OVA-KP. The right lung was removed for RNA isolation. Each group contained between 4 and 5 mice.
Contributor(s) Dulek D, Goleniewska K, Peebles S
Citation(s) 24958709
Submission date May 13, 2014
Last update date May 15, 2019
Contact name Daniel E Dulek
Organization name Vanderbilt University Medical Center
Department Pediatrics
Lab Stokes Peebles, PI
Street address D-7235 Medical Center North, 1161 21st Avenue South
City Nashville
State/province TN
ZIP/Postal code 37232-2581
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (8)
GSM1385424 WT-ALUM
GSM1385425 WT-OVA
BioProject PRJNA246779
SRA SRP041929

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Supplementary file Size Download File type/resource
GSE57602_KOalum0_vs_KOova0_gene_exp_significant.diff.gz 2.4 Kb (ftp)(http) DIFF
GSE57602_KOalum6_vs_KOova6_gene_exp_significant.diff.gz 2.1 Kb (ftp)(http) DIFF
GSE57602_WTalum0_vs_WTova0_gene_exp_significant.diff.gz 21.5 Kb (ftp)(http) DIFF
GSE57602_WTalum6_vs_WTova6_gene_exp_significant.diff.gz 7.6 Kb (ftp)(http) DIFF
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