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Series GSE57526 Query DataSets for GSE57526
Status Public on Jan 23, 2017
Title Expression data from TGRL lipolysis products treated human brain microvascular endothelial cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Lipotoxicity is a metabolic disorder that results from accumulation of lipids, particularly fatty acids, in non-adipose tissue leading to cellular dysfunction, lipid droplet formation, and cell death. Our studies indicate for the first time that the neurovascular circulation also can manifest lipotoxicity, which could have major effects on cognitive function. The penetration of integrative systems biology approaches is limited in this area of research, which reduces our capacity to gain an objective insight into the signal transduction and regulation dynamics at a systems level.
We used microarrays to determine the TGRL lipolysis products induced gene expression and to understand the mechanism underline the vascular inflammation.
Overall design Total RNA was isolated from human brain microvascular endothelial cells treated with media (M), Lipoprotein lipase (L), triglyceride-rich lipoprotein (TGRL or T), or TGRL lipolysis products (TL) treatment. Affymetrix microarrays analyses of the pooled total RNA samples (n = 3 per group for Media, LpL, TGRL, and TGRL lipolysis products treatments) were performed.
Contributor(s) Aung HH, Tsoukalas A, Rutledge JC, Tagkopoulos I
Citation(s) 24993133
Submission date May 09, 2014
Last update date Mar 22, 2021
Contact name Hnin H Aung
Phone 530-752-2182
Organization name University of California, Davis
Department Internal Medicine
Street address 451 E Health Science Dr., GBSF 5404
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (4)
GSM1384230 Media, biological rep1 (3 pooled replicates)
GSM1384231 LpL, biological rep1 (3 pooled replicates)
GSM1384232 TGRL, biological rep1 (3 pooled replicates)
BioProject PRJNA246593

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57526_RAW.tar 7.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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