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| Status |
Public on Feb 16, 2015 |
| Title |
Transcriptome wide annotation of eukaryotic RNase III reactivity and degradation signals [Expression 2] |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
Here we present complete genomic and biochemical annotations of the signals required for RNA degradation by the dsRNA specific ribonuclease III (Rnt1p) and examine its impact on transcriptome expression. Rnt1p cleavage signals are randomly distributed in the yeast genome and encompass wide variety of sequence indicating that transcriptome stability is not determined by the recurrence of a fixed cleavage motif. Instead, RNA reactivity is defined by the sequence and structural context in which the cleavage sites are located. Reactive signals are often associated with transiently expressed genes and their impact on RNA expression is linked to growth conditions. Together the data suggest that Rnt1p reactivity is triggered by malleable RNA degradation signals that permit dynamic response to changes in growth conditions.
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| Overall design |
Total RNA and whole cell extract from rnt1d strain was divided in two samples. One sample was treated with purified Rnt1p. The other was only incubated with buffer. The two samples were then treated with recombinant Xrn1p.
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| Contributor(s) |
Gagnon J, Lavoie M, Catala M, Malenfant F, Elela SA |
| Citation(s) |
25680180 |
| |
| Submission date |
May 08, 2014 |
| Last update date |
Jan 09, 2016 |
| Contact name |
Sherif Abou Elela |
| E-mail(s) |
sherif.abou.elela@usherbrooke.ca
|
| Organization name |
Université de Sherbrooke Centre of Excellence in RNA Biology
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| Department |
Microbiologie and Infectiologie
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| Lab |
Abou Elela
|
| Street address |
3201 Jean Mignault
|
| City |
Sherbrooke |
| State/province |
Québec |
| ZIP/Postal code |
J1E 4K8 |
| Country |
Canada |
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| Platforms (1) |
| GPL7250 |
[Sc03b_MR] Affymetrix GeneChip S. cerevisiae Tiling 1.0R Array |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE57450 |
Transcriptome Wide Annotation of Eukaryotic RNase III Reactivity and Degradation Signals |
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| Relations |
| BioProject |
PRJNA246476 |
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