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Series GSE57392 Query DataSets for GSE57392
Status Public on May 11, 2015
Title ChIP-seq profiling of H3K27 tri-methylation in Ezh2-deficient and control murine lung epithelium at day E16.5
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Epithelial (CD31-CD45-EpCAM+) lung cells were derived by FACS from ShhCre;Ezh2fl/fl and control ShhCre;Ezh2fl/+ mouse embryos at day E16.5 (2 biological replicates per genotype). Chromatin from each of the replicates was immunoprecipitated with H3K27me3 antibody (Millipore #07-449) and subjected to NGS library preparation using TruSeq Nano DNA Sample Preparation Kit (Illumina). Completed libraries from different samples were sequenced on HiSeq 2500 TruSeq with SBS Kit v3 - HS reagents (Illumina) as 100 bp single end reads at the Australian Genome Research Facility.
Overall design ChIP-seq profiles of H3K27 tri-methylation from Ezh2 deficient and control lung epithelium (2 replicates per genotype).
Contributor(s) Holik AZ
Citation(s) 25790853, 26484284
Submission date May 07, 2014
Last update date Jun 07, 2019
Contact name Marie-Liesse Asselin-Labat
Organization name The Walter and Eliza Hall Institute of Medical Research
Department Stem Cells and Cancer
Street address 1G Royal Parade
City Parkville
State/province VIC
ZIP/Postal code 3052
Country Australia
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (4)
GSM1381952 Control Epithelium rep1
GSM1381953 Control Epithelium rep2
GSM1381954 Ezh2KO Epithelium rep1
This SubSeries is part of SuperSeries:
GSE57393 Repression of Igf1 expression by Ezh2 prevents basal cell differentiation in the developing lung
BioProject PRJNA246383
SRA SRP041750

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Supplementary file Size Download File type/resource
GSE57392_binned.10kb.txt.gz 2.4 Mb (ftp)(http) TXT
GSE57392_binned.2kb.txt.gz 9.8 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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