GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE57379 Query DataSets for GSE57379
Status Public on May 01, 2015
Title Insights into the proliferation and invasiveness of breast cancer from genome-wide profiling of estrogen receptor beta 2
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The human ERβ variant, ERβ2, is expressed at higher levels than ERβ1 in many breast cancer cell lines and breast tumours and increasing evidence supports the hypothesis that ERβ2, in contrast to ERβ1, is associated with aggressive phenotypes of various cancers. We identified a breast cancer cell line that expresses endogenous ERβ2, but not ERα or ERβ1, allowing studies of ERβ2 in the absence of other ER isoforms. Importantly, we show that knockdown of endogenous ERβ2 inhibited cell proliferation and cell invasion. To gain insights into the molecular mechanism responsible for these cellular phenotypes, we assayed ERβ2 dependent global gene expression profiles. A total of 263 genes were identified as potentially ERβ2-upregulated genes and 662 as ERβ2-downregulated genes. ERβ2-regulated genes were involved in cell morphology, DNA replication and repair, cell death and survival. Our findings demonstrate that ERβ2 alone has a key role in enhancing cell proliferation and invasion, beyond modulation of ERα and ERβ1 signalling.
Overall design BT549 cells grown in antibiotic free medium were transfected with a control siRNA, siRNA targeting ERβ2, ERβ2 cDNA, empty vector control, and then global gene expression profiles were assessed. Three biological replicates were used for each group.
Contributor(s) Xu L, Zhao C, Gustafsson J, Dahlman-Wright K
Citation(s) 29100336
Submission date May 07, 2014
Last update date May 24, 2019
Contact name Li Xu
Organization name Karolinska Institute
Department Biosciences and Nutrition
Street address Hälsovägen 7-9
City Stockholm
ZIP/Postal code 141 83
Country Sweden
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (12)
GSM1381451 BT549_sicontrol_rep1
GSM1381452 BT549_sicontrol_rep2
GSM1381453 BT549_sicontrol_rep3
BioProject PRJNA246367

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57379_RAW.tar 55.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap