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Series GSE57318 Query DataSets for GSE57318
Status Public on Jun 29, 2015
Title Novel culture conditions facilitating the induction of naïve pluripotency in human embryonic stem cells
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary We report a novel culture condition inducing naive pluripotency in hESCs in a rapid, robust and reproducible way. These naive hESCs were similar to mESCs exhibiting domed colony morphology, increased single survival, reduced doubling time, upregulation of naive pluripotency-specific genes, unbiased lineage-specific differentiation, hypomethylation, separate clustering profile from parental primed hESCs and were dependent on signalling pathways similar to naive mESCs.
Overall design Global DNA methylation analysis of naive hESCs and their parental primed hESC counterparts.
Contributor(s) Duggal G, Ghimire S, Brokaert D, Deroo T, Van der Jeught M, Warrier S, Lierman S, Peelman L, Van Soom A, Menten B, Mestdagh P, Vandesompele J, Roost M, Slieker RC, Heijmans BT, Deforce D, De Sutter P, Chuva De Sousa Lopes S, Heindryckx B
Citation(s) 26108678
Submission date May 05, 2014
Last update date Mar 22, 2019
Contact name Nannan He
Organization name Leiden University Medical Center
Street address Einthovenweg 20
City Leiden
ZIP/Postal code 2333 ZC
Country Netherlands
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (6)
GSM1379487 n-UGent11-2, rep1
GSM1379488 n-UGent11-2, rep2
GSM1379489 n-H1 OCT4:GFP, rep1
BioProject PRJNA246240

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57318_RAW.tar 183.1 Mb (http)(custom) TAR
GSE57318_methylated_unmethylated_signals_after_quality_control.txt.gz 15.1 Mb (ftp)(http) TXT
GSE57318_methylated_unmethylated_signals_unfiltered.txt.gz 16.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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