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Series GSE57248 Query DataSets for GSE57248
Status Public on Nov 30, 2015
Title Gfi1 proteins regulate the emergence of HSCs and blood precursors in the AGM through recruitment of LSD1 [HTS]
Organism Mus musculus
Experiment type Other
Summary Gfi1:Dam and Gfi1b:Dam binding analysis of sorted hemogenic endothelial cells (TIE2/C-KIT)
Overall design The cDNAs encoding DAM-GFI1 and DAM-GFI1B fusion proteins or a DAM control were inserted in a Dox inducible HPRT docking site in ainv18 ES cells (Kyba et al, 2002). Following differentiation, HE cell populations were isolated, genomic DNA prepared and digested with DpnI. The methylated DNA fragments were amplified as previously described {Orian et al, 2009, Vogel et al, 2007) and sequenced on a Illumina HiSeq platform
Contributor(s) Thambyrajah RS, Mazan M, Patel R, Moignard V, Stefanska M, Clapes T, Möröy T, Robin C, Cowley S, Göttgens B, Kouskoff V, Lacaud G
Citation(s) 26619147
Submission date May 02, 2014
Last update date May 15, 2019
Contact name Roshana S Thambyrajah
Organization name CRUK Manchester
Department Stem Cell Biology
Street address Wilmslow Road
City Manchester
ZIP/Postal code M20 4BX
Country United Kingdom
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (3)
GSM1377856 HE_DAM ctrl
GSM1377857 HE_GFI1:DAM
This SubSeries is part of SuperSeries:
GSE57251 Gfi1 proteins regulate the emergence of HSCs and blood precursors in the AGM through recruitment of LSD1
BioProject PRJNA246690
SRA SRP041873

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Supplementary file Size Download File type/resource
GSE57248_rthambyrajah_Gfi1damID_and_Gfi1bdamID_gene_lists.xlsx.gz 677.5 Kb (ftp)(http) XLSX
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Processed data are available on Series record
Raw data are available in SRA

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