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Series GSE57237 Query DataSets for GSE57237
Status Public on Jun 01, 2014
Title Gene expression in the pancreatic lymph nodes (PLN) of hyperglycemic 16-week-old Non-Obese Diabetic (NOD) mice compared to expression the PLN of euglycemic 16-week-old NOD mice.
Organism Mus musculus
Experiment type Expression profiling by array
Summary These experiments were performed to identify differentially expressed genes in the pancreatic lymph nodes of hyperglycemic NOD mice with high levels of destructive insulitis compared to euglycemic mice with lower levels of insulitis.
 
Overall design The pancreatic lymph nodes of 16-week-old hyperglycemic and euglycemic NOD mice were isolated and homogenized in Trizol reagent (Invitrogen). Total RNA was extracted from the aqueous phase using the RNeasy mini kit (Qiagen). RNA quality was assessed using the Agilent 2100 Bioanalyzer and the RNA 6000 Nano Reagent Kit (Agilent). Total RNA from the PLN of hyperglycemic mice (n=2) were labelled with Cy5 and run individually against a pool of Cy3-labeled total RNA isolated from euglycemic mice (n=2). Microarrays were performed using the Whole Mouse Genome Microarray Kit, 4×44K 2-color arrays (Agilent Technologies).
 
Contributor(s) Yip L
Citation(s) 25187368
Submission date May 01, 2014
Last update date Jan 12, 2017
Contact name Linda Yip
Organization name Stanford University
Department Medicine
Lab C.G. Fathman
Street address 269 Campus Drive West, CCSR Building Rm. 2240
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (2)
GSM1377694 Hyperglycemic 16 week old NOD PLN - sample 1
GSM1377695 Hyperglycemic 16 week old NOD PLN - sample 2
Relations
BioProject PRJNA246029

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57237_RAW.tar 28.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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