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Series GSE57222 Query DataSets for GSE57222
Status Public on Aug 01, 2014
Title NSD3-NUT Fusion Oncoprotein in NUT Midline Carcinoma identified in 1221 patient-derived cell line by RNA-seq
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Discarded live tumor tissue from a metastatic focus in the patient’s lung was collected under institutional review board approval through the NUT midline carcinoma registry ( From this tissue the first known NUT-variant cell line, 1221, was established. To determine the putative partner gene to NUT, we performed comprehensive RNA-sequencing on RNA purified from 1221. We identified an in-frame transcript fusing the 5’ coding sequence of NSD3 (exons 1-7) to exons 2-7 of NUT. Expression of the NSD3-NUT fusion oncoprotein was verified by immunobloting with an antibody to NUT, revealing an approximately 200kDa band that is similar in size to BRD3-NUT, but smaller than BRD4-NUT
Overall design Identification of a NUT fusion partner using RNA extracted from live cultured 1221 cell line derived from a lung metastasis from the index case of a 13 year old female with NUT-positive NMC.
Contributor(s) Walsh EM, Lemieux ME, French CA
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Submission date Apr 30, 2014
Last update date May 15, 2019
Contact name Christopher A French
Organization name Brigham and Women's Hospita
Department Pathology
Lab French Lab
Street address 77 Avenue Louis Pasteur 630G
City Boston
State/province Massachusetts
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (1)
GSM1377430 1221 cell line
BioProject PRJNA245923
SRA SRP041594

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Supplementary file Size Download File type/resource
GSE57222_RAW.tar 20.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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