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| Status |
Public on Aug 01, 2014 |
| Title |
NSD3-NUT Fusion Oncoprotein in NUT Midline Carcinoma identified in 1221 patient-derived cell line by RNA-seq |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Discarded live tumor tissue from a metastatic focus in the patient’s lung was collected under institutional review board approval through the NUT midline carcinoma registry (www.NMCRegistry.org). From this tissue the first known NUT-variant cell line, 1221, was established. To determine the putative partner gene to NUT, we performed comprehensive RNA-sequencing on RNA purified from 1221. We identified an in-frame transcript fusing the 5’ coding sequence of NSD3 (exons 1-7) to exons 2-7 of NUT. Expression of the NSD3-NUT fusion oncoprotein was verified by immunobloting with an antibody to NUT, revealing an approximately 200kDa band that is similar in size to BRD3-NUT, but smaller than BRD4-NUT
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| Overall design |
Identification of a NUT fusion partner using RNA extracted from live cultured 1221 cell line derived from a lung metastasis from the index case of a 13 year old female with NUT-positive NMC.
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| Contributor(s) |
Walsh EM, Lemieux ME, French CA |
| Citation missing |
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| |
| Submission date |
Apr 30, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Christopher A French |
| E-mail(s) |
cfrench@partners.org
|
| Organization name |
Brigham and Women's Hospita
|
| Department |
Pathology
|
| Lab |
French Lab
|
| Street address |
77 Avenue Louis Pasteur 630G
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA245923 |
| SRA |
SRP041594 |
