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Series GSE57221 Query DataSets for GSE57221
Status Public on Sep 12, 2016
Title ChIP-seq of E4F1 in mouse embryonic stem cells (mESCs)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary E4F1 is a ubiquitously expressed zinc-finger protein of the Gli-Kruppel family that was first identified, more than 30 years ago, as a cellular target of the adenoviral oncoprotein E1A13S (Ad type V), required for transcriptional regulation of adenoviral genes. In order to decipher E4F1 cellular target genes, we performed chromatin immunoprecipitation of endogenous E4F1 in mouse embryonic stem cells. Both input and immunoprecipitated DNA were exhaustively sequenced and mapped on the mouse genome (mm9). Peak detection has been achieved by combining two peak calling algorithms. Intersection of the two E4F1 peak lists on each cell line were considered as E4F1 chromatin bound regions.
Overall design Genome-wide mapping of E4F1 binding in mouse embryonic stem cells.
Contributor(s) Kirsh O, Rodier G, Sardet C
Citation(s) 27621446, 27621431
Submission date Apr 30, 2014
Last update date May 15, 2019
Contact name Claude Sardet
Organization name Institut de Recherche en Cancérologie de Montpellier (IRCM)
Department Inserm U1194 - Université de Montpellier - Institut Régional du Cancer de Montpellier (ICM)
Street address 208 rue des Apothicaires
City Montpellier
ZIP/Postal code 34298
Country France
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (2)
GSM1377428 E4F1 ChIP in mESC
GSM1377429 mESC input
BioProject PRJNA246034
SRA SRP041608

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57221_RAW.tar 139.0 Mb (http)(custom) TAR (of BAR, BED, COD, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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