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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 12, 2016 |
| Title |
ChIP-seq of E4F1 in mouse embryonic stem cells (mESCs) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
E4F1 is a ubiquitously expressed zinc-finger protein of the Gli-Kruppel family that was first identified, more than 30 years ago, as a cellular target of the adenoviral oncoprotein E1A13S (Ad type V), required for transcriptional regulation of adenoviral genes. In order to decipher E4F1 cellular target genes, we performed chromatin immunoprecipitation of endogenous E4F1 in mouse embryonic stem cells. Both input and immunoprecipitated DNA were exhaustively sequenced and mapped on the mouse genome (mm9). Peak detection has been achieved by combining two peak calling algorithms. Intersection of the two E4F1 peak lists on each cell line were considered as E4F1 chromatin bound regions.
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| Overall design |
Genome-wide mapping of E4F1 binding in mouse embryonic stem cells.
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| Contributor(s) |
Kirsh O, Rodier G, Sardet C |
| Citation(s) |
27621446, 27621431 |
| Submission date |
Apr 30, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Claude Sardet |
| E-mail(s) |
claude.sardet@inserm.fr
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| Organization name |
Institut de Recherche en Cancérologie de Montpellier (IRCM)
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| Department |
Inserm U1194 - Université de Montpellier - Institut Régional du Cancer de Montpellier (ICM)
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| Street address |
208 rue des Apothicaires
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| City |
Montpellier |
| ZIP/Postal code |
34298 |
| Country |
France |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA246034 |
| SRA |
SRP041608 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE57221_RAW.tar |
139.0 Mb |
(http)(custom) |
TAR (of BAR, BED, COD, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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