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| Status |
Public on Feb 11, 2017 |
| Title |
Expression profile after stable HIF-1a inhibition in gastric cancer cells under normoxic conditions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Based on the results of numerous clinical and preclinical analyses, the transcription factor HIF-1a has been identified as an important tumor-promoting factor and is considered to be an attractive target for cancer therapy. To further deconstruct the molecular nature of HIF-1a’s role in tumorigenesis, we have applied lentiviral shRNA transduction to establish HIF-1a-deficient gastric cancer cells. Interestingly, functional analyses failed to show a significant growth defect of HIF-1a-deficient gastric cancer cells in vitro and in vivo. These observations led us to propose that stable inactivation of HIF-1a resulted in efficient compensation enabling cell growth and, ultimately, tumor progression in a HIF-1a-independent manner. To better understand the mechanisms that control this compensation, we performed transcriptomics of control (“scrambled” (SCR)) and HIF-1a-deficient (HIF) gastric cancer cells.
Analysis of hypoxia-inducible factor-1alpha (HIF-1a)-deficient gastric cancer cells under normoxia. The transcription factor HIF-1a is a key regulator of oxygen homeostasis and has been identified as an important tumor-promoting factor. Results provide insight into the role of HIF-1a in gastric carcinogenesis.
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| Overall design |
Gastric cancer AGS cells were lentiviral and stably transduced with a small hairpin RNA targeting human HIF-1a or a control shRNA. RNA was extracted from control and HIF-1a-deficient cells. Each cell line was analyzed in triplicate for a total of six samples.
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| Contributor(s) |
Rohwer N, Bindel F, Grimm C, Lehrach H, Klinger B, Blüthgen N, Du Bois I, Schmeck B, de Graauw M, Prigione A, Kempa S, Cramer T |
| Citation(s) |
26760764 |
| Submission date |
Apr 30, 2014 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Nadine Rohwer |
| E-mail(s) |
nadine.rohwer@charite.de
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| Organization name |
Charité
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| Street address |
Augustenburger Platz 1
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| City |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
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| Platforms (2) |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA245899 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE57200_RAW.tar |
30.1 Mb |
(http)(custom) |
TAR |
| GSE57200_non-normalized_rep1_2.txt.gz |
836.6 Kb |
(ftp)(http) |
TXT |
| GSE57200_non-normalized_rep3.txt.gz |
2.0 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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