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Series GSE56623 Query DataSets for GSE56623
Status Public on Apr 09, 2014
Title RNAseq to investigate transcriptional changes in human MM cell lines due to panobinostat, 5-Azacytidine, panobinostat+5-Azacytidine or n-methyl-2-pyrroldine (NMP) treatments.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: We applied RNA sequencing technology for high-throughput analysis of transcriptional changes within human MM cell lines JJN3 and U266 due to individual and combination drug treatment.
Methods: JJN3 and U266 cells were treated with pan-HDACi panbobinostat, DNMTi 5-Azacytidine, panobinostat+5-Azacytidine or NMP for 4h or 24h in triplicate and transcriptional changes assessed by RNAseq using Illumina HiSeq platform. Specifically, JJN3 cells were treated with 10nM panobinostat, 2.5µM 5-Azacytidine, panobinostat+5-Azacytidine (at given doses), or 10mM NMP. U266 cells were treated with 10nM panobinostat, 10µM 5-Azacytidine, panobinostat+5-Azacytidine (at given doses), or 10mM NMP.
Results: We report unique and overlapping transcriptional signatures that lead to the induction of apoptosis in human MM cell lines in a cell-specific manner due to individual or combination treatments.
Conclusions: A detailed analysis of differential transcriptional events in human MM cell lines due to HDACi, DNMTi, HDACi+DNMTi and NMP appear to define the molecular events leading to apoptosis and drug mechanism of action.
Overall design We tested triplicate experiments at 4h and 24hr time points in JJN3 and U266 cell lines against vehicle control treated cells.
Contributor(s) Matthews GM, Shortt J
Citation(s) 24030150, 24813887
Submission date Apr 08, 2014
Last update date May 15, 2019
Contact name Geoffrey Mark Matthews
Phone +61396563724
Organization name Peter MacCallum Cancer Centre
Department Cancer Therapeutics
Lab Gene Regulation
Street address St Andrews Place
City Melbourne
State/province Victoria
ZIP/Postal code 3002
Country Australia
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (60)
GSM1365932 1.4hr.JJN3.Control
GSM1365933 2.4hr.JJN3.LBH.589
GSM1365934 3.4hr.JJN3.5.AZA
BioProject PRJNA244098
SRA SRP041036

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Supplementary file Size Download File type/resource
GSE56623_AZA_LBH_Combo_NMP_normalised_expression_matrix.txt.gz 7.7 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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