|
| Status |
Public on Apr 01, 2014 |
| Title |
Understanding molecular mechanisms of Cetuximab insensitivity in Colorectal Cancer (CRC) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Mutational status of KRAS in CRC is used to aid patient stratification for Cetuximab treatment. However, only a subset (10-40%) of patients with wt KRAS respond. We analyzed 40 mCRC tumors for Cetuximab response using a functional ex vivo platform. In the subset of non-responsive tumors, mutational (KRAS/BRAF and PIK3CA) and expression (AREG/EREG) analysis of key genes, transcriptomic profiling and GSEA were carried out to elucidate the molecular mechanisms underlying the response. Our analysis revealed deregulation of multiple pathways, notably Notch and Erbb2 and combined blockade of these two nodes elicited significant antitumor response. These findings collectively indicate the dependence of Cetuximab insensitive mCRC tumors on Notch and Erbb2 for survival and progression.
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| |
|
| Overall design |
8 Primary tumors tested in ex vivo platform for response to Cetuximab were subjected to expression analysis
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| |
|
| Contributor(s) |
Brijwani N |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 31, 2014 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Pradip K Majumder |
| Organization name |
Mitra Biotech Pvt Ltd.
|
| Street address |
Suite 202, Narayana Nethralaya, Narayana Hrudayalaya Health City, Hosur Main Road, Bangalore 99, Karnataka State.
|
| City |
Bangalore |
| ZIP/Postal code |
560099 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
|
| Samples (8)
|
| GSM1360149 |
Primary CRC tumor responder to Cetuximab in ex vivo platform 1 |
| GSM1360150 |
Primary CRC tumor responder to Cetuximab in ex vivo platform 2 |
| GSM1360151 |
Primary CRC tumor responder to Cetuximab in ex vivo platform 3 |
| GSM1360152 |
Primary CRC tumor responder to Cetuximab in ex vivo platform 4 |
| GSM1360153 |
Primary CRC tumor non responder to Cetuximab in ex vivo platform 1 |
| GSM1360154 |
Primary CRC tumor non responder to Cetuximab in ex vivo platform 2 |
| GSM1360155 |
Primary CRC tumor non responder to Cetuximab in ex vivo platform 3 |
| GSM1360156 |
Primary CRC tumor non responder to Cetuximab in ex vivo platform 4 |
|
| Relations |
| BioProject |
PRJNA243086 |
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