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Series GSE56343 Query DataSets for GSE56343
Status Public on Mar 29, 2014
Title Next-generation sequencing facilitates quantitative analysis of AcKLF5 and unAcKLF5 transcriptomes in xenografts of DU-145 cell line
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary KLF5 possesses both tumor-suppressing and tumor-promoting activities, though the mechanism controlling these opposing functions is unknown. In cultured non-cancerous epithelial cells, KLF5 converts from pro-proliferative to anti-proliferative activity upon TGFβ-induced acetylation, which sequentially alters the KLF5 transcriptional complex and the expression of genes such as p15 and MYC. In nude mice, KLF5 also suppressed tumor growth in an acetylation-dependent manner. Furthermore, deacetylation switched KLF5 to tumor-promoting activity, and blocking TGFβ signaling attenuated the tumor suppressor activity of KLF5. The results from RNA-Seq indicated the different downstream genes of AcKLF5 and unAcKLF5 and the mechanisms that determine the opposing functions of AcKLF5 and unAcKLF5. These results provide novel insights into the mechanism by which KLF5 switches from anti-tumorigenic to pro-tumorigenic function, and also suggest the roles of AcKLF5 and unAcKLF5, respectively, in the tumor-suppressing and tumor-promoting functions of TGFβ.
Overall design Xenografts of DU-145 cell lines expressing KLF5, KLF5 mutant K369R or the empty vector PLHCX were surgically isolated from mice at day 38 after injection. RNA-Seq was performed using these three groups of xenografts.
Contributor(s) Dong JT, Li X, Zhang B, Wu Q, Ci X, Zhao R, Zhang Z, Xia S, Su D, Chen J, Ma G, Fu L
Citation(s) 24931571
Submission date Mar 28, 2014
Last update date May 15, 2019
Contact name jintang dong
Organization name Nankai university
Street address Weijin road
City Tianjin
ZIP/Postal code 300071
Country China
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (3)
GSM1359516 K369R
GSM1359517 KLF5
GSM1359518 PLHCX
BioProject PRJNA242967
SRA SRP040692

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Supplementary file Size Download File type/resource
GSE56343_gene_expression.txt.gz 1.3 Mb (ftp)(http) TXT
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Processed data are available on Series record

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