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Series GSE56256 Query DataSets for GSE56256
Status Public on Mar 14, 2017
Title Influence of hexafluoro-2-propanol (HFIP) on lipopolysaccharides-induced inflammation in human microvascular endothelial cells of the lung
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Hexafluoro-2-propanol (HFIP), a water-soluble primary metabolite of the volatile anesthetic sevoflurane, has recently been shown to have favorable properties, improving overall survival in a chronic model of murine peritonitis. The mechanisms of protection provided by HFIP are currently unknown.
We therefore performed a gene expression analysis in an in vitro model of endotoxin-induced endothelial cell injury to gain insight into involved molecular mechanisms. Pathway analysis was subsequently performed using the GeneGo Metacore Framework.
Overall design Human microvascular endothelial cells (HMVEC) were stimulated with 20mcg/mL lipopolysaccharides (LPS) to induce an inflammatory response. Hexafluoro-2-propanol (HFIP) was added to the cell culture medium containing LPS (phosphate-buffered saline, PBS, in controls) for a time period of 6 hours at a concentration of 8mmol/L. After 6 hous, total RNA from HMVEC was extracted and genome-wide RNA levels were assessed. In total, twelve samples with LPS- or PBS-stimulated HMVEC, from which six samples were treated with HFIP, were hybridized on microarrays . One sample of the LPS+HFIP group was lost due to technical reasons (hybridization procedure).
Contributor(s) Urner M, Herrmann IK, Hasler M, Schimmer RR, Booy C, Z’graggen BR, Rehrauer H, Schläpfer M, Aigner F, Minshall RD, Stark WJ, Beck-Schimmer B
Citation(s) 25925908
Submission date Mar 26, 2014
Last update date Nov 27, 2018
Contact name Beatrice Beck-Schimmer
Phone 0041 44 635 50 35
Organization name University of Zurich
Department Institute of Physiology
Lab Group Beck-Schimmer
Street address Winterthurerstrasse 190
City Zurich
ZIP/Postal code 8057
Country Switzerland
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (11)
GSM1357752 7_HFIP
GSM1357753 8_HFIP
GSM1357754 9_HFIP
BioProject PRJNA242764

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE56256_RAW.tar 39.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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