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| Status |
Public on Mar 14, 2017 |
| Title |
Influence of hexafluoro-2-propanol (HFIP) on lipopolysaccharides-induced inflammation in human microvascular endothelial cells of the lung |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Hexafluoro-2-propanol (HFIP), a water-soluble primary metabolite of the volatile anesthetic sevoflurane, has recently been shown to have favorable properties, improving overall survival in a chronic model of murine peritonitis. The mechanisms of protection provided by HFIP are currently unknown.
We therefore performed a gene expression analysis in an in vitro model of endotoxin-induced endothelial cell injury to gain insight into involved molecular mechanisms. Pathway analysis was subsequently performed using the GeneGo Metacore Framework.
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| Overall design |
Human microvascular endothelial cells (HMVEC) were stimulated with 20mcg/mL lipopolysaccharides (LPS) to induce an inflammatory response. Hexafluoro-2-propanol (HFIP) was added to the cell culture medium containing LPS (phosphate-buffered saline, PBS, in controls) for a time period of 6 hours at a concentration of 8mmol/L. After 6 hous, total RNA from HMVEC was extracted and genome-wide RNA levels were assessed. In total, twelve samples with LPS- or PBS-stimulated HMVEC, from which six samples were treated with HFIP, were hybridized on microarrays . One sample of the LPS+HFIP group was lost due to technical reasons (hybridization procedure).
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| Contributor(s) |
Urner M, Herrmann IK, Hasler M, Schimmer RR, Booy C, Z’graggen BR, Rehrauer H, Schläpfer M, Aigner F, Minshall RD, Stark WJ, Beck-Schimmer B |
| Citation(s) |
25925908 |
| Submission date |
Mar 26, 2014 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Beatrice Beck-Schimmer |
| E-mail(s) |
Beatrice.BeckSchimmer@uzh.ch
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| Phone |
0041 44 635 50 35
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| Organization name |
University of Zurich
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| Department |
Institute of Physiology
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| Lab |
Group Beck-Schimmer
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| Street address |
Winterthurerstrasse 190
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| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA242764 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE56256_RAW.tar |
39.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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