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| Status |
Public on Aug 26, 2006 |
| Title |
Comparison of gene expression by co-cultured WC1+ gammadelta and CD4+ alphabeta T cells |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by array
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| Summary |
Vaccination of animals with leptospiral vaccine results in the generation of antigen-responsive CD4 alphabeta T cells and WC1 gammadelta T cells which proliferate and produce effector cytokines following secondary encounter with antigen. In these studies we compared the gene expression of these antigen-stimulated T cell subsets to each other by microarray. In addition, each activated T cell population was compared to a reference sample, consisting of unstimulated peripheral blood mononuclear cells.
Keywords: cell type comparison
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| Overall design |
CD4 T cells from 3 vaccinated animals compared to WC1 T cells from same animals, following in vitro antigen-stimulation. Each array was repeated for each animal using dye-swapped samples.
CD4 or WC1 T cells from each vaccinated animal were also compared to ex vivo PBMC (unstimulated control). Here sample from antigen-stimulated CD4 or WC1 T cell was always Cy3 labelled, while unstimulated control was always Cy5 labelled.
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| Contributor(s) |
Blumerman S, Herzig C, Wang F, Coussens P, Baldwin C |
| Citation(s) |
17081609 |
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| Submission date |
Aug 24, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Seth Blumerman |
| E-mail(s) |
namersb@yahoo.com
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| Organization name |
University of Massachusetts, Amherst
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| Street address |
Paige Laboratory
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| City |
Amherst |
| State/province |
MA |
| ZIP/Postal code |
01002 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| GSM130275 |
CD4 alphabeta T cell vs WC1 gammadelta T cell |
| GSM130276 |
WC1 gammadelta T cell vs CD4 alphabeta T cell |
| GSM130775 |
CD4 alphabeta T cell vs WC1 gammadelta T cell, #2 |
| GSM130776 |
WC1 gammadelta T cell vs CD4 alphabeta T cell, #2 |
| GSM130777 |
CD4 alphabeta T cell vs WC1 gammadelta T cell, #3 |
| GSM130778 |
WC1 gammadelta T cell vs CD4 alphabeta T cell, #3 |
| GSM130779 |
CD4 alphabeta T cell vs reference |
| GSM130780 |
CD4 alphabeta T cell vs reference, #2 |
| GSM130781 |
CD4 alphabeta T cell vs reference, #3 |
| GSM130782 |
WC1 gammadelta T cell vs Reference |
| GSM130783 |
WC1 gammadelta T cell vs Reference, #2 |
| GSM130784 |
WC1 gammadelta T cell vs Reference, #3 |
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| Relations |
| BioProject |
PRJNA96889 |
| Supplementary data files not provided |
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