GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE55886 Query DataSets for GSE55886
Status Public on Sep 01, 2014
Title Effect of Spot14 Loss on Gene Expression Profile of MMTV-PyMT Mouse Tumors
Organism Mus musculus
Experiment type Expression profiling by array
Summary The objective of this study was to determine the effect of Thyroid Hormone Responsive Protein Spot14 (Spot14) loss on the gene expression profiles of tumors from MMTV-Polyomavirus middle-T antigen (PyMT) mice. MMTV-PyMT/S14-heterozygous mice were crossed with S14-heterozygous mice and 1 cm tumors from MMTV-PyMT control (wild-type S14) or MMTV-PyMT/S14-null offspring were profiled using Affymetrix gene arrays. Tumor latency was not different between groups; however, tumors lacking S14 grew significantly slower than control tumors. Loss of S14 also decreased the levels of de novo synthesized fatty acids in mammary tumors. In additional studies, performed on MMTV-Neu mice, we found that S14 overexpression was associated with increased tumor cell proliferation and elevated levels of tumor fatty acids. Gene expression profiling revealed that S14 loss and overexpression in mouse mammary tumors altered pathways associated with proliferation and metabolism. This study provides important information about the role of S14 in mammary tumorigenesis and tumor metabolism.
Overall design Microarray analysis was performed on 4 mammary tumors from MMTV-PyMT mice and 4 tumors from MMTV-PyMT/S14-null mice.
Contributor(s) Anderson SM, Wellberg EA
Citation(s) 25472762
Submission date Mar 13, 2014
Last update date Apr 18, 2017
Contact name Elizabeth A Wellberg
Organization name University of Oklahoma HSC
Department Pathology
Street address 975 NE 10th St, BRC 309
City Oklahoma City
State/province Oklahoma
ZIP/Postal code 73104
Country USA
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (8)
GSM1347955 PyMT Tumor A
GSM1347956 PyMT Tumor B
GSM1347957 PyMT Tumor C
BioProject PRJNA241245

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE55886_RAW.tar 35.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap