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| Status |
Public on Dec 31, 2016 |
| Title |
Comparative gene expression profiling of cutaneous B cell lymphomas |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The oncogenic microRNA 17-92 cluster is involved in the lymphomagenesis of nodal and systemic B cell lymphomas, but has not been studied so far in primary cutaneous B cell lymphomas. We performed the quantitative analysis of miR-17-92 cluster and its paralogs miR-106a-363 and miR-106b-25 in 22 primary cutaneous diffuse large B-cell lymphomas-leg type (PCLBCL-LT) and 22 primary cutaneous follicle center lymphomas (PCFCL). Mir-20a and miR-106a were overexpressed in PCLBCL-LT compared to PCFCL. Multivariate Cox analysis showed that higher miR-20a and miR-20b were associated with poorer disease-free survival and poorer overall survival, independently of histological type. Gene expression profiling showed underexpression of 8 candidate target genes of miR-20a, miR-20b and miR-106a in PCLBCL-LT compared to PCFCL. Among the candidate target genes, PTEN, NCOA3 and CAPRIN2 were confirmed to be underexpressed in PCLBCL-LT using quantitative RT-PCR of laser-microdissected tumor cells. In multivariate Cox analysis, lower PTEN mRNA expression level was associated with poorer disease-free survival, independently of the histological type. Altogether, this molecular and bioinformatics study of patients’ skin biopsy samples showed that the oncogenic miR-17-92 cluster is involved in cutaneous B cell lymphoma progression, and that the down-regulation of its target gene PTEN is associated with poorer disease-free survival.
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| Overall design |
10 skin samples from patients were analyzed. 6 were PCLBCL-leg type and 4 PCFCL.
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| Contributor(s) |
Battistella M, Romero M, Castro Vega L, Gapihan G, Bouhidel F, Bachelez H, Feugeas J, Janin A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 12, 2014 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Anne Janin |
| E-mail(s) |
jean-paul.feugeas@sls.aphp.fr
|
| Phone |
+33142494012
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| Organization name |
University Paris 7, INSERM, APHP
|
| Street address |
1 Av C Vellefaux
|
| City |
Paris |
| ZIP/Postal code |
75010 |
| Country |
France |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA241106 |
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