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Series GSE55833 Query DataSets for GSE55833
Status Public on Dec 31, 2016
Title Comparative gene expression profiling of cutaneous B cell lymphomas
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The oncogenic microRNA 17-92 cluster is involved in the lymphomagenesis of nodal and systemic B cell lymphomas, but has not been studied so far in primary cutaneous B cell lymphomas. We performed the quantitative analysis of miR-17-92 cluster and its paralogs miR-106a-363 and miR-106b-25 in 22 primary cutaneous diffuse large B-cell lymphomas-leg type (PCLBCL-LT) and 22 primary cutaneous follicle center lymphomas (PCFCL). Mir-20a and miR-106a were overexpressed in PCLBCL-LT compared to PCFCL. Multivariate Cox analysis showed that higher miR-20a and miR-20b were associated with poorer disease-free survival and poorer overall survival, independently of histological type. Gene expression profiling showed underexpression of 8 candidate target genes of miR-20a, miR-20b and miR-106a in PCLBCL-LT compared to PCFCL. Among the candidate target genes, PTEN, NCOA3 and CAPRIN2 were confirmed to be underexpressed in PCLBCL-LT using quantitative RT-PCR of laser-microdissected tumor cells. In multivariate Cox analysis, lower PTEN mRNA expression level was associated with poorer disease-free survival, independently of the histological type. Altogether, this molecular and bioinformatics study of patients’ skin biopsy samples showed that the oncogenic miR-17-92 cluster is involved in cutaneous B cell lymphoma progression, and that the down-regulation of its target gene PTEN is associated with poorer disease-free survival.
Overall design 10 skin samples from patients were analyzed. 6 were PCLBCL-leg type and 4 PCFCL.
Contributor(s) Battistella M, Romero M, Castro Vega L, Gapihan G, Bouhidel F, Bachelez H, Feugeas J, Janin A
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Submission date Mar 12, 2014
Last update date Nov 27, 2018
Contact name Anne Janin
Phone +33142494012
Organization name University Paris 7, INSERM, APHP
Street address 1 Av C Vellefaux
City Paris
ZIP/Postal code 75010
Country France
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (10)
GSM1346896 skin-PCLBCLLT-1
GSM1346897 skin-PCLBCLLT-2
GSM1346898 skin-PCLBCLLT-3
BioProject PRJNA241106

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE55833_RAW.tar 128.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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