GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE55605 Query DataSets for GSE55605
Status Public on Jul 06, 2014
Title Role of Polycomb group proteins in the DNA damage response – a reassessment
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We have investigated whether components of Polycomb repressive complex 1 (PRC1) are recruited to double-strand breaks (DSBs) generated by inducible expression of the AsiSI restriction enzyme in normal human fibroblasts. Using chromatin immunoprecipitation and deep sequencing (ChIP-seq), which detects PRC1 proteins at common sites throughout the genome, we did not find evidence for recruitment of PRC1 components to AsiSI-induced DSBs. In contrast, the S2056 phosphorylated form of DNA-PKcs and other DNA repair proteins were detected at a subset of AsiSI sites that are predominantly at the 5’ ends of transcriptionally active genes. Our data question the idea that PcG protein recruitment provides a link between DSB repairs and transcriptional repression.
Overall design Single chromatin preparations from treated and untreated cells (+/- OHT), immunoprecipitated with two antibodies (pDNA-PKcs and MEL18) were sequenced as technical replicates, along with the corresponding input DNAs
Contributor(s) Chandler H, Patel H, Palermo R, Brookes S, Matthews N, Peters G
Citation(s) 25057768
Submission date Mar 05, 2014
Last update date May 15, 2019
Contact name Gordon Peters
Phone +44 207 269 3049
Organization name CRUK London Research Institute
Lab Molecular Oncology
Street address 44 Lincolns Inn Fields
City London
ZIP/Postal code WC2A 3LY
Country United Kingdom
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (6)
GSM1340089 DNAPKCS METHANOL HS ChipSeq technical replicate 1 and 2
GSM1340090 DNAPKCS OHT HS ChipSeq technical replicate 1 and 2
GSM1340091 MEL18 METHANOL HS ChipSeq technical replicate 1 and 2
BioProject PRJNA240224
SRA SRP039445

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE55605_RAW.tar 3.0 Gb (http)(custom) TAR (of BED, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap