|
| Status |
Public on Jul 23, 2015 |
| Title |
Transcription factor Runx1 cooperates with MyoD and c-Jun to regulate the balance of myoblast proliferation/differentiation [Expression Study] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Runx1 is expressed in regenrating muscle, specifically in the muscle adult stem cells- the satellite cells. Its exact role and target genes were yet to be identified
This experiment aimed at determining the Runx1 regulated genes in muscle regeneration, specifically in Satellite cells
|
| |
|
| Overall design |
To elucidate the Runx1-mediated transcriptional program involved in early stages of muscle regeneration, we compared gene expression profiles in WT and Runx1f/f primary myobalsts (PM). PM were purified from both genotypes , Total RNA was isolated, reverse-transcribed, fragmented, labeled and hybridized to Affymetrix MoGene 1.0 ST DNA array.
|
| |
|
| Contributor(s) |
Umansky K, Gruenbaum-Choen Y, Feldmesser E, Horn-Saban S, Goldenberg D, Groner Y |
| Citation(s) |
26697350 |
| |
| Submission date |
Mar 04, 2014 |
| Last update date |
Jul 31, 2019 |
| Contact name |
kfir baruch Umansky |
| E-mail(s) |
kfirum@weizmann.ac.il
|
| Phone |
97289342318
|
| Organization name |
Weizmann Institute
|
| Street address |
POB 25
|
| City |
Rehovot |
| State/province |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (8)
|
| GSM1339124 |
WT primary myoblasts, biological replicate 1 |
| GSM1339125 |
WT primary myoblasts, biological replicate 2 |
| GSM1339126 |
WT primary myoblasts, biological replicate 3 |
| GSM1339127 |
WT primary myoblasts, biological replicate 4 |
| GSM1339128 |
Runx1f/f primary myoblasts, biological replicate 1 |
| GSM1339129 |
Runx1f/f primary myoblasts, biological replicate 2 |
| GSM1339130 |
Runx1f/f primary myoblasts, biological replicate 3 |
| GSM1339131 |
Runx1f/f primary myoblasts, biological replicate 4 |
|
| This SubSeries is part of SuperSeries: |
| GSE56131 |
Transcription factor Runx1 cooperates with MyoD and c-Jun to regulate the balance of myoblast proliferation/differentiation |
|
| Relations |
| BioProject |
PRJNA239959 |
Less...
More...