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| Status |
Public on Mar 05, 2014 |
| Title |
Gene expression profiling of HPV-active oropharyngeal cancers and Normal benign uvula, tonsil |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Head and neck cancer (HNC) is the fifth most common malignancy worldwide with an annual mortality rate of 200,000. About 90% of HNC can be classified as head and neck squamous cell carcinomas (HNSCC), of which approximately 75% are attributed to alcohol and tobacco consumption and 25 are associated with human papillomavirus (HPV), predominantly HPV16. HPV-associated OPC have better prognosis and a more favorable response to therapy as compared to HPV-negative tumors. Viral oncoproteins are capable of transforming primary human keratinocytes from either genital or oral epithelia in vitro and most likely play the same role in vivo, by disrupting cell-cycle regulatory pathways leading to a genetic progression to ano-genital cancer and OPC. However, the precise mechanisms by which HPV mediates malignant transformation of keratinocytes in the upper digestive tract epithelia are not entirely clear. HPV E7-mediated inactivation of pRb results in overexpression of p16INK4A, which is commonly used as a clinical surrogate marker for HPV positivity/activity. However, high p16INK4A alone has insufficient sensitivity and specificity as a biomarker of HPV positivity in different mucosal sub-sites of HNC. Therefore, increasing emphasis is being placed on the assessment of viral load and E7 oncogene expression, resulting in further classification of HPV positive OPC as HPV-active and HPV-inactive. Differences in risk factors, age of presentation, clinical behavior and gene expression profiles indicate that HPV-positive and HPV-negative tumors develop via different molecular mechanisms and are biologically distinct. This study aimed to compare the gene expression profiles of HPV-active OPCs and normal benign uvula/tonsil tissues and determine what biological processes and pathways are affected in HPV-active tumors.
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| Overall design |
ANALYSIS 5: Two-condition, one-color experiment: HPV-active oropharyngeal tumor samples and normal benign uvula/tonsil tissues. Biological replicates: 12 HPV active samples and 4 Normal samples.
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| Contributor(s) |
Tomar S, Altomare D, Kowli S, Kassler S, Sutkowski N, Gillespie MB, Creek KE, Pirisi L |
| Citation(s) |
25899179 |
| |
| Submission date |
Mar 04, 2014 |
| Last update date |
Apr 23, 2018 |
| Contact name |
Diego Altomare |
| Organization name |
University of South Carolina
|
| Department |
Department of Drug Discovery and Biomedical Sciences
|
| Lab |
Functional Genomics Core
|
| Street address |
715 Sumter Street, Room 617
|
| City |
Columbia |
| State/province |
SC |
| ZIP/Postal code |
29208 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (16)
|
| GSM1339029 |
Analysis 5: HPV Active - Biological replicate 1 |
| GSM1339030 |
Analysis 5: HPV Active - Biological replicate 2 |
| GSM1339031 |
Analysis 5: HPV Active - Biological replicate 3 |
| GSM1339032 |
Analysis 5: HPV Active - Biological replicate 4 |
| GSM1339033 |
Analysis 5: HPV Active - Biological replicate 5 |
| GSM1339034 |
Analysis 5: HPV Active - Biological replicate 6 |
| GSM1339035 |
Analysis 5: HPV Active - Biological replicate 7 |
| GSM1339036 |
Analysis 5: HPV Active - Biological replicate 8 |
| GSM1339037 |
Analysis 5: HPV Active - Biological replicate 9 |
| GSM1339038 |
Analysis 5: HPV Active - Biological replicate 10 |
| GSM1339039 |
Analysis 5: HPV Active - Biological replicate 11 |
| GSM1339040 |
Analysis 5: HPV Active - Biological replicate 12 |
| GSM1339041 |
Analysis 5: Normal - Biological replicate 1 |
| GSM1339042 |
Analysis 5: Normal - Biological replicate 2 |
| GSM1339043 |
Analysis 5: Normal - Biological replicate 3 |
| GSM1339044 |
Analysis 5: Normal - Biological replicate 4 |
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| This SubSeries is part of SuperSeries: |
| GSE55550 |
Gene expression profiles of oral and oropharyngeal cancers from European American and African American patients |
|
| Relations |
| BioProject |
PRJNA239998 |
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