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Series GSE55495 Query DataSets for GSE55495
Status Public on Mar 11, 2014
Title Effect of ultradian light-dark cycles on the transcriptome of mice carying a human Per3 polymorphism
Organism Mus musculus
Experiment type Expression profiling by array
Summary In humans, a primate-specific variable-number tandem-repeat (VNTR) polymorphism (4 or 5 repeats 54 nt in length) in the circadian gene PER3 is associated with differences in sleep timing and homeostatic responses to sleep loss. We investigated the effects of this polymorphism on circadian rhythmicity and sleep homeostasis by introducing the polymorphism into mice and assessing circadian and sleep parameters at baseline and during and after 12 h of sleep deprivation (SD). Microarray analysis was used to measure hypothalamic and cortical gene expression. Circadian behavior and sleep were normal at baseline. The response to SD of 2 electrophysiological markers of sleep homeostasis, electroencephalography (EEG) θ power during wakefulness and δ power during sleep, were greater in the Per35/5 mice. During recovery, the Per35/5 mice fully compensated for the SD-induced deficit in δ power, but the Per34/4 and wild-type mice did not. Sleep homeostasis-related transcripts (e.g., Homer1, Ptgs2, and Kcna2) were differentially expressed between the humanized mice, but circadian clock genes were not. These data are in accordance with the hypothesis derived from human data that the PER3 VNTR polymorphism modifies the sleep homeostatic response without significantly influencing circadian parameters.-Hasan, S., van der Veen, D. R., Winsky-Sommerer, R., Hogben, A., Laing, E. E., Koentgen, F., Dijk, D.-J., Archer, S. N. A human sleep homeostasis phenotype in mice expressing a primate-specific PER3 variable-number tandem-repeat coding-region polymorphism.
Overall design Mice were kept under 3.5h light- 3.5 hours dark cycles and samples were collected in the 17th light cycle
Boxhill represents Per35/5 mice and Coach represents Per34/4 mice.
A total of 30 samples comprizing 30 mice
Contributor(s) van der Veen DR, Hasan S, Winsky-Sommerer R, Hogeben A, Laing E, Koentgen F, Dijk D, Archer SN
Citation(s) 24577121
Submission date Mar 02, 2014
Last update date Jan 12, 2017
Contact name Emma Laing
Organization name University of Surrey
Street address Stag Hill
City Guildford
ZIP/Postal code GU22 7XH
Country United Kingdom
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (30)
GSM1338199 Brain_Hypothalamus_Ultradian_Boxhill_R1
GSM1338200 Brain_Hypothalamus_Ultradian_Boxhill_R2
GSM1338201 Brain_Hypothalamus_Ultradian_Boxhill_R3
This SubSeries is part of SuperSeries:
GSE55255 A human sleep homeostasis phenotype in mice expressing a primate-specific PER3 variable-number tandem-repeat coding-region polymorphism
BioProject PRJNA239783

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE55495_RAW.tar 160.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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