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Series GSE55428 Query DataSets for GSE55428
Status Public on Feb 09, 2017
Title Effect of FGFC on human embryonic stem cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Fibroblast growth factors (FGFs) are essential for maintaining self-renewal in human embryonic stem cells and induced pluripotent stem cells. Recombinant basic FGF (bFGF or FGF2) is conventionally used to culture pluripotent stem cells; however, because of bFGF instability, repeated addition of fresh bFGF into the culture medium is required in order to maintain its concentration. In this study, we demonstrate that a heat-stable chimeric variant of FGF, FGFC, can be successfully used for maintaining human pluripotent stem cells. FGFC is a chimeric protein composed of human FGF1 and FGF2 domains that exhibits higher thermal stability and protease resistance than do both FGF1 and FGF2. Both human embryonic stem cells and induced pluripotent stem cells were maintained in ordinary culture medium containing FGFC instead of FGF2. Comparison of cells grown in FGFC with those grown in conventional FGF2 media, showed no significant differences in terms of the expression of pluripotency markers, global gene expression, karyotype, or differentiation potential into the three germ lineages. We therefore propose FGFC, as an effective alternative to FGF2, for maintenance of human pluripotent stem cells.
Overall design Gene expressions in human ES and iPS cells were measured at 4-50 days cultured with FGFC, in place of bFGF.
Contributor(s) Onuma Y, Ito Y
Citation(s) 25850016
Submission date Feb 27, 2014
Last update date Nov 27, 2018
Contact name Yuzuru Ito
Organization name National Institute of Advanced Industrial Science and Technology (AIST)
Department Biotechnology Research Institute for Drug Discovery
Street address Central 6, Higashi 1-1-1
City Tsukuba
State/province Ibaraki
ZIP/Postal code 3058566
Country Japan
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (54)
GSM1336461 WA01 (H1)_p48_p_exp1
GSM1336462 WA01 (H1)_p48_p_exp1_2
GSM1336463 WA01 (H1)_p49_4_bFGF_exp1
BioProject PRJNA239631

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE55428_RAW.tar 173.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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