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Series GSE55404 Query DataSets for GSE55404
Status Public on Sep 26, 2015
Title TG-interacting factor1 (Tgif1) maintains the identity of mouse ES cells by counterbalancing the expression of ES cell core factors
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary TG-interacting factor1 (Tgif1) is well-known as a transcriptional repressor in transforming growth factor beta (TGFβ) signaling pathway. Target mapping of ES cell core factors in mouse embryonic stem (ES) cells revealed that Tgif1 is occupied by Oct4 and Nanog. Moreover, recent interactome study of mouse gene regulatory regions showed a preferential regulation of Tgif1 by mouse ES cell specific enhancers. However, the detailed role and mode of actions of Tgif1 in stem cell maintenance and development remains elusive. We show that Tgif1 is indispensable for self-renewal and pluripotency of mouse embryonic stem (ES) cells. Aberrant expression of Tgif1 promotes differentiation of ES cells even in the presence of LIF in part by deregulation of pluripotency factors. Intriguingly, we find that Tgif1 level is a critical factor to determine specific lineage commitment in a dosage-dependent manner. We further show that Tgif1 interacts with ES cell core factors and co-localizes at their binding sites, which eventually restricts expression of ES cell core factors including Oct4, Sox2, and Nanog. Taken together, we provide new insights into the roles of Tgif1 in maintenance as well as differentiation of ES cells.
Overall design ChIP sequenicngs were performed for 5 transcription factors (Tgif1, Tgif2, Nanog, Hdac1 and Smad3) in mouse ES cells. Nanog ChIP was performed using bioChIP-seq. Control ChIP-seq was performed using BirA cells.
Contributor(s) Kim J
Citation(s) 26411691
Submission date Feb 26, 2014
Last update date May 15, 2019
Contact name Bum-Kyu Lee
Phone 512-232-8046
Organization name University of Texas at Austin
Street address 2506 Speedway Stop A5000
City Austin
State/province TX
ZIP/Postal code 78712
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM1335481 Tgif1_ChIPSeq
GSM1335482 HDAC1_ChIPSeq
GSM1335483 Smad3_ChIPseq
This SubSeries is part of SuperSeries:
GSE55437 TG-interacting factor1 (Tgif1) maintains the identity of mouse ES cells by counterbalancing the expression of core pluripotency factors and ES cell core factors
BioProject PRJNA239579
SRA SRP039028

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Supplementary file Size Download File type/resource
GSE55404_RAW.tar 1.3 Mb (http)(custom) TAR (of TXT)
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Processed data provided as supplementary file
Raw data are available in SRA

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