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Series GSE55394 Query DataSets for GSE55394
Status Public on May 15, 2015
Title mCasz1_conditional knockout
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The overall goal of our studies is to elucidate the cellular and molecular mechanism by which the transcription factor Casz1 functions in murine heart development. We established that Casz1 is expressed in myocardial progenitor cells beginning at E7.5 and in differentiated cardiomyocytes throughout development. We generated conditional Casz1 knockout mice to show that ablation of CASZ1 in Nkx2.5-positive cardiomyocytes leads to cardiac hypoplasia, ventricular septal defects and lethality by E13.5. To identify the pathways and networks by which Casz1 regulates cardiomyocyte development, we used RNA-Seq and identified genes involved in cell proliferation are upregulated in Casz1 mutants compared to wild-type littermates. We conclude that Casz1 is essential for cardiac development and has a pivotal role in regulating part of the cardiac transcriptional program.
Overall design 3 biological replicates of the two genotypes (Nkx2-5+/+,Casz1f/+ and Nkx2-5Cre/+,Casz1f/f) were used for RNA-seq to determine genes that are differentially expressed in the murine heart when Casz1 is mutated. Nkx2-5+/+,Casz1f/+ were used as wild-type controls for comparison.
Contributor(s) Dorr KM, Amin NM, Conlon FL
Citation(s) 25953344
Submission date Feb 26, 2014
Last update date May 15, 2019
Contact name Nirav M Amin
Phone 9195157403
Organization name North Carolina State University
Department Molecular Biomedical Sciences
Lab Nascone-Yoder
Street address 1060 William Moore Drive
City Raleigh
State/province NC
ZIP/Postal code 27607
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM1335300 wild-type1
GSM1335301 mutant1
GSM1335302 mutant2
BioProject PRJNA239462
SRA SRP038989

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Supplementary file Size Download File type/resource
GSE55394_gene_exp.diff.txt.gz 933.8 Kb (ftp)(http) TXT
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