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Series GSE55263 Query DataSets for GSE55263
Status Public on Sep 04, 2014
Title Genome wide occupancy of Msgn1 in differentiating embryonic stem cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Msgn1 is a bHLH transcription factor and is a direct target gene of the Wnt/b-catenin signaling pathway. During mouse embryogenesis, Msgn1 is expressed in the mesodermal compartment of the primitive streak and is required for the differentiation of presomitic mesoderm. Msgn1-/- mutants show defects in somitogenesis leading to a lack of trunk skeletal muscles, vertebra and ribs. The goal of this study is to dissect the molecular and cellular function of Msgn1 in Embryonic Stem Cells (ESC) and mouse development. In order to identify direct Msgn1 targets, we performed transcriptional profiling and CHIP-seq of Msgn1 expressing differentiating ES cells. Integration of these data sets, we found that Msgn1 is a master regulator of PSM differentiation regulating gene expression programs of PSM identity, EMT, motility and Notch segmentation clock.
Overall design Inducible Flag Msgn1 ES cells were differentiated to form Embryoid bodies (EBs) for 2 days. Flag-Msgn1 was induced on day 2 with doxycycline and samples were collected 36h later. Here Input DNA is used as control.
Contributor(s) Chalamalasetty RB, Yamaguchi TP
Citation(s) 25371364
Submission date Feb 21, 2014
Last update date May 15, 2019
Contact name Ravindra B Chalamalasetty
Phone 3018466507
Organization name National Cancer Institute-Frederick
Department Cancer and Developmental Biology
Lab Cell Signaling in Vertebrate Development Section
Street address 1050 Boyles St, Bldg 539,Rm205
City Frederick
State/province MD
ZIP/Postal code 21702
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (2)
GSM1332768 FMsgn1_Chipseq
GSM1332769 FMsgn1_Input DNA
BioProject PRJNA239012
SRA SRP038754

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Supplementary file Size Download File type/resource
GSE55263_FLAG_vs_Input_4087peaks.txt.gz 59.7 Kb (ftp)(http) TXT
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