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| Status |
Public on Sep 01, 2014 |
| Title |
Alternative Capture of Noncoding RNAs or Protein-Coding Genes by Herpesviruses to Alter Host T-Cell Function |
| Organism |
Callithrix jacchus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In marmoset T cells transformed by Herpesvirus saimiri (HVS), a viral U-rich noncoding RNA, HSUR 1, specifically mediates degradation of host microRNA-27 (miR-27). High-throughput sequencing of RNA after crosslinking immunoprecipitation (HITS-CLIP) identified mRNAs targeted by miR-27 as enriched in the T-cell receptor (TCR) signaling pathway, including GRB2. Accordingly, transfection of miR-27 into human T cells attenuates TCR-induced activation of mitogen-activated protein kinases (MAPKs) and induction of CD69. MiR-27 also robustly regulates SEMA7A and IFN-γ, key modulators and effectors of T-cell function. Knockdown or ectopic expression of HSUR 1 alters levels of these proteins in virally-transformed cells. Two other T-lymphotropic γ-herpesviruses, AlHV-1 and OvHV-2, do not produce a noncoding RNA to downregulate miR-27, but instead encode homologs of miR-27 target genes. Thus, oncogenic γ-herpesviruses have evolved diverse strategies to converge on common targets in host T cells.
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| Overall design |
HVS-transformed marmoset T cells were transfected with ASOs against HSUR 1 or control, or with LNAs against miR-27 or control, in replicates; poly A+ RNAs were selected and sequenced on HiSeq 2000.
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| Contributor(s) |
Guo YE, Riley KJ, Iwasaki A, Steitz JA |
| Citation(s) |
24725595 |
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| Submission date |
Feb 19, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Yang Eric Guo |
| E-mail(s) |
eric.guo@wi.mit.edu
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| Organization name |
Whitehead Institute MIT
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| Lab |
Young
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| Street address |
455 Main St
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (2) |
| GPL17712 |
Illumina HiSeq 2000 (Callithrix jacchus) |
| GPL18304 |
Illumina Genome Analyzer II (Callithrix jacchus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA238829 |
| SRA |
SRP038206 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE55185_H1_KD_WT.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
| GSE55185_RAW.tar |
220.0 Kb |
(http)(custom) |
TAR (of TXT) |
| GSE55185_mR27_KD_2A.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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